Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLEKHO1 / CKIP-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112754-100
Catalog Number: LS-G112754
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHO1 (NM_016274), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CK2-interacting protein 1 | CKIP1 | JBP | OC120 | PLEKHO1 | C-Jun-binding protein | CKIP-1 | RP11-458I7.3
- Origin Species
- Human
- NCBI GenBank
- NM_016274
- SwissProt
- Q53GL0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG DQLYISEKEV61KDEKNIQEVF DLSDYEKCEE LRKSKSRSKK NHSKFTLAHS KQPGNTAPNL IFLAVSPEEK121ESWINALNSA ITRAKNRILD EVTVEEDSYL AHPTRDRAKI QHSRRPPTRG HLMAVASTST181SDGMLTLDLI QEEDPSPEEP TSCAESFRVD LDKSVAQLAG SRRRADSDRI QPSADRASSL241SRPWEKTDKG ATYTPQAPKK LTPTEKGRCA SLEEILSQRD AASARTLQLR AEEPPTPALP301NPGQLSRIQD LVARKLEETQ ELLAEVQGLG DGKRKAKDPP RSPPDSESEQ LLLETERLLG361EASSNWSQAK RVLQEVRELR DLYRQMDLQT PDSHLRQTTP HSQYRKSLMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
409 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.