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PLEKHN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109016-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHN1 (NM_032129), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434H2010 | RP11-54O7.7 | PLEKHN1
Origin Species
Human
NCBI GenBank
NM_032129

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MGNSHCVPQA PRRLRASFSR KPSLKGNRED SARMSAGLPG PEAARSGDAA ANKLFHYIPG
61TDILDLENQR ENLEQPFLSV FKKGRRRVPV RNLGKVVHYA KVQLRFQHSQ DVSDCYLELF
121PAHLYFQAHG SEGLTFQGLL PLTELSVCPL EGSREHAFQI TGPLPAPLLV LCPSRAELDR
181WLYHLEKQTA LLGGPRRCHS APPQRRLTRL RTASGHEPGG SAVCASRVKL QHLPAQEQWD
241RLLVLYPTSL AIFSEELDGL CFKGELPLRA VHINLEEKEK QIRSFLIEGP LINTIRVVCA
301SYEDYGHWLL CLRAVTHREG APPLPGAESF PGSQVMGSGR GSLSSGGQTS WDSGCLAPPS
361TRTSHSLPES SVPSTVGCSS QHTPDQANSD RASIGRRRTE LRRSGSSRSP GSKARAEGRG
421PVTPLHLDLT QLHRLSLESS PDAPDHTSET SHSPLYADPY TPPATSHRRV TDVRGLEEFL
481SAMQSARGPT PSSPLPSVPV SVPASDPRSC SSGPAGPYLL SKKGALQSRA AQRHRGSAKD
541GGPQPPDAPQ LVSSAREGSP EPWLPLTDGR SPRRSRDPGY DHLWDETLSS SHQKCPQLGG
601PEASGGLVQW I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

611 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.