Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLEKHM2 / SKIP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113423-100
Catalog Number: LS-G113423
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHM2 (NM_015164), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PLEKHM2 | RP11-169K16.1 | SKIP | KIAA0842
- Origin Species
- Human
- NCBI GenBank
- AB020649
- SwissProt
- Q8IWE5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 112.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPGEVKDRI LENISLSVKK LQSYFAACED EIPAIRNHDK VLQRLCEHLD HALLYGLQDL61SSGYWVLVVH FTRREAIKQI EVLQHVATNL GRSRAWLYLA LNENSLESYL RLFQENLGLL121HKYYVKNALV CSHDHLTLFL TLVSGLEFIR FELDLDAPYL DLAPYMPDYY KPQYLLDFED181RLPSSVHGSD SLSLNSFNSV TSTNLEWDDS AIAPSSEDYD FGDVFPAVPS VPSTDWEDGD241LTDTVSGPRS TASDLTSSKA STRSPTQRQN PFNEEPAETV SSSDTTPVHT TSQEKEEAQA301LDPPDACTEL EVIRVTKKKK IGKKKKSRSD EEASPLHPAC SQKKCAKQGD GDSRNGSPSL361GRDSPDTMLA SPQEEGEGPS STTESSERSE PGLLIPEMKD TSMERLGQPL SKVIDQLNGQ421LDPSTWCSRA EPPDQSFRTG SPGDAPERPP LCDFSEGLSA PMDFYRFTVE SPSTVTSGGG481HHDPAGLGQP LHVPSSPEAA GQEEEGGGGE GQTPRPLEDT TREAQELEAQ LSLVREGPVS541EPEPGTQEVL CQLKRDQPSP CLSSAEDSGV DEGQGSPSEM VHSSEFRVDN NHLLLLMIHV601FRENEEQLFK MIRMSTGHME GNLQLLYVLL TDCYVYLLRK GATEKPYLVE EAVSYNELDY661VSVGLDQQTV KLVCTNRRKQ FLLDTADVAL AEFFLASLKS AMIKGCREPP YPSILTDATM721EKLALAKFVA QESKCEASAV TVRFYGLVHW EDPTDESLGP TPCHCSPPEG TITKEGMLHY781KAGTSYLGKE HWKTCFVVLS NGILYQYPDR TDVIPLLSVN MGGEQCGGCR RANTTDRPHA841FQVILSDRPC LELSAESEAE MAEWMQHLCQ AVSKGVIPQG VAPSPCIPCC LVLTDDRLFT901CHEDCQTSFF RSLGTAKLGD ISAVSTEPGK EYCVLEFSQD SQQLLPPWVI YLSCTSELDR961LLSALNSGWK TIYQVDLPHT AIQEASNKKK FEDALSLIHS AWQRSDSLCR GRASRDPWCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1019 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.