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PLEKHH3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHH3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109656-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHH3 (NM_024927), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PLEKHH3
Origin Species
Human
NCBI GenBank
NM_024927
SwissProt
Q7Z736

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLPGGLWWL LCCRRGFTLL HRDYGDGELS GDGDEDEDEE TFELRTPSPA GGGRGPLEVT
61LTQPVRSGPV SNRLQSWEET WSLIPEKGLP EDDPDIVVKG WLYREPRGGG ARPWLPPRRA
121WFVLTRDSLD QFSSSGKGAR RLGSLVLTSL CSVTGPERRR KETGLWSVTV SGRKHSVRLC
181SPRQAEAERW GVALREVIAS KAPLETPTQL LLRDIQESCG DPEAVALIYL RNPILRHTSG
241ALYAPLLPLP YGVSAPGPGY APLREEAVRL FLALQALEGA RRPGPLMQGV LQTCRDLPAL
301RDELFLQLAK QTSGPAGPPG LPATQDPAAL RYWQLLTCMS CTFRPGGAVR GHLLGHLERT
361EQALPDSELA EYARFIRKAL GRTRGRELVP SLAEISALSQ RQELLCTVHC PGAGACAVAI
421DSHTTAGEVA RELVGRLGLA RSRNAFALYE QRGAQERALA GGTLVADVLT RFENLAAEEA
481GLEDSPDSGW RLCLRLHGPL HPEGLSPDGH ELPFLFEQAH ALLLRGRPPP PDDTLRALAA
541LRLQSLHRDF SPRVPLPRLD RLLPPPAPPR EDPPRPTPRP PPSAALLAGA LWSPGLAKRR
601AERARRGGAG RTAGSIAREG GGGAGTAAAV LGGWKRLRGM GRAEAMAAYL ALAAQCPGFG
661AARYDVLELS TEPGRGAPQK LCLGLGAKAM SLSRPGETEP IHSVSYGHVA ACQLMGPHTL
721ALRVGESQLL LQSPQVEEIM QLVNAYLANP SPERPCSSSS PPCQDLPDTS PPSQRPGLDE
781PQGQSGCLGQ LQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

793 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.