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PLEKHH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLEKHH2 antibodies →
$725.00each
Catalog Number: LS-G105548-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHH2 (NM_172069), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA2028 | PLEKHH1L | PLEKHH2
Origin Species
Human
SwissProt
Q8IVE3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
168 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELSEPEGP VDWKERCVAL ESQLMKFRVQ ASKIRELLAE KMQQLERQVI DAERQAEKAF
61QQVQVMEDKL KAANIQTSES ETRLYNKCQD LESLIQEKDD VIQNLELQLE EQKQIRIQEA
121KIIEEKAAKI KEWVTVKLNE LELENQNLRL INQNQTEEIR TMQSKLQEVQ GKKSSTVSTL
181KLSEGQRLSS LTFGCFLSRA RSPPQVVKSE EMSKISSKEP EFTEGKDMEE MEIPEKSVDN
241QVLENNRGQR TLHQTPCGSE QNRKTRTSFA TDGGISQNSG APVSDWSSDE EDGSKGRSKS
301RCTSTLSSHT SEEGVQCSRM GSEMYLTASD DSSSIFEEET FGIKRPEHKK LYSWQQEAQW
361KALNSPLGKG NSELSKKEQD SSSDELNKKF QSQRLDYSSS SSEANTPSPI LTPALMPKHP
421NSLSGKGTQL VPSSHLPPPK LRIPNVFSIS AALAKRHLSQ PQLSSDRMFG TNRNAISMIR
481PLRPQETDLD LVDGDSTEVL ENMDTSCDDG LFSYDSLDSP NSDDQEHCDS AKKVAYSKPP
541TPPLHRFPSW ESRIYAVAKS GIRMSEAFNM ESVNKNSAAT LSYTTSGLYT SLIYKNMTTP
601VYTTLKGKAT QISSSPFLDD SSGSEEEDSS RSSSRTSESD SRSRSGPGSP RAMKRGVSLS
661SVASESDYAI PPDAYSTDTE YSQPEQKLPK TCSSSSDNGK NEPLEKSGYL LKMSGKVKSW
721KRRWFVLKGC ELLYYKSPSD VIRKPQGHIE LSASCSILRG DNKQTVQLTT EKHTYYLTAD
781SPNILEEWIK VLQNVLRVQA ANPLSLQPEG KPTMKGLLTK VKHGYSKRVW CTLIGKTLYY
841FRSQEDKFPL GQIKLWEVKV EEVDRSCDSD EDYEASGRSL LSTHYTIVIH PKDQGPTYLL
901IGSKHEKDTW LYHLTVAAGS NNVNVGSEFE QLVCKLLNID GEPSSQIWRH PTLCHSKEGI
961ISPLTTLPSE ALQTEAIKLF KTCQLFINAA VDSPAIDYHI SLAQSALQIC LTHPELQNEI
1021CCQLIKQTRR RQPQNQPGPL QGWQLLALCV GLFLPHHPFL WLLRLHLKRN ADSRTEFGKY
1081AIYCQRCVER TQQNGDREAR PSRMEILSTL LRNPYHHSLP FSIPVHFMNG IYQVVGFDAS
1141TTVEEFLNTL NQDTGMRKPA QSGFALFTDD PSGRDLEHCL QGNIKICDII SKWEQASKEQ
1201QPGKCEGTRT VRLTYKNRLY FSVQARGETD REKLLLMYQT NDQIINGLFP LNKDLALEMA
1261ALLSQVEIGD FERPFSTPAG HVTNQCKVNQ TLKQVIEKFY PKRYRDGCSE EQLRQLCQRL
1321STRWMALRGH SAADCVRIYL TVARKWPFFG AKLFLAKPIT PSSLGSTFLW LAVHEDGLSL
1381LEYNSMRLIV SYVYKSLMTF GGYQDDFMVV INNTHSKDKP TEKLLFAMAK PKILEITLLI
1441ASYINNFHQQ KAAFHHLSAP ALLSAQTRGP QARMMGSQPL LSSSRPTKGP TLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.