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PLEKHG6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHG6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G126363-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MyoGEF(PLEKHG6) (NM_001144857), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MyoGEF | PLEKHG6
Origin Species
Human
NCBI GenBank
NM_018173
SwissProt
Q3KR16

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCRLHAPGE KAAHDPSRRR LQQYVPFARG SGQARGLSPM RLRDPEPEKR HGGHVGAGLL
61HSPKLKELTK AHELEVRLHT FSMFGMPRLP PEDRRHWEIG EGGDSGLTIE KSWRELVPGH
121KEMSQELCHQ QEALWELLTT ELIYVRKLKI MTDLLAAGLL NLQRVGLLME VSAETLFGNV
181PSLIRTHRSF WDEVLGPTLE ETRASGQPLD PIGLQSGFLT FGQRFHPYVQ YCLRVKQTMA
241YAREQQETNP LFHAFVQWCE KHKRSGRQML CDLLIKPHQR ITKYPLLLHA VLKRSPEARA
301QEALNAMIEA VESFLRHING QVRQGEEQES LAAAAQRIGP YEVLEPPSDE VEKNLRPFST
361LDLTSPMLGV ASEHTRQLLL EGPVRVKEGR EGKLDVYLFL FSDVLLVTKP QRKADKAKVI
421RPPLMLEKLV CQPLRDPNSF LLIHLTEFQC VSSALLVHCP SPTDRAQWLE KTQQAQAALQ
481KLKAEEYVQQ KRELLTLYRD QDRESPSTRP STPSLEGSQS SAEGRTPEFS TIIPHLVVTE
541DTDEDAPLVP DDTSDSGYGT LIPGTPTGSR SPLSRLRQRA LRRDPRLTFS TLELRDIPLR
601PHPPDPQAPQ RRSAPELPEG ILKGGSLPQE DPPTWSEEED GASERGNVVV ETLHRARLRG
661QLPSSPTHAD SAGESPWESS GEEEEEGPLF LKAGHTSLRP MRAEDMLREI REELASQRIE
721GAEEPRDSRP RKLTRAQLQR MRGPHIIQLD TPLSASEV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

758 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.