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PLEKHG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119620-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHG5 (NM_001042664), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DSMA4 | NFKB activating protein | PLEKHG5 | KIAA0720 | GEF720 | Syx | Tech
Origin Species
Human
NCBI GenBank
AK024676

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHYDGHVRFD LPPQGSVLAR NVSTRSCPPR TSPAVDLEEE EEESSVDGKG DRKSTGLKLS
61KKKARRRHTD DPSKECFTLK FDLNVDIETE IVPAMKKKSL GEVLLPVFER KGIALGKVDI
121YLDQSNTPLS LTFEAYRFGG HYLRVKAPAK PGDEGKVEQG MKDSKSLSLP ILRPAGTGPP
181ALERVDAQSR RESLDILAPG RRRKNMSEFL GEASIPGQEP PTPSSCSLPS GSSGSTNTGD
241SWKNRAASRF SGFFSSGPST SAFGREVDKM EQLEGKLHTY SLFGLPRLPR GLRFDHDSWE
301EEYDEDEDED NACLRLEDSW RELIDGHEKL TRRQCHQQEA VWELLHTEAS YIRKLRVIIN
361LFLCCLLNLQ ESGLLCEVEA ERLFSNIPEI AQLHRRLWAS VMAPVLEKAR RTRALLQPGD
421FLKGFKMFGS LFKPYIRYCM EEEGCMEYMR GLLRDNDLFR AYITWAEKHP QCQRLKLSDM
481LAKPHQRLTK YPLLLKSVLR KTEEPRAKEA VVAMIGSVER FIHHVNACMR QRQERQRLAA
541VVSRIDAYEV VESSSDEVDK LLKEFLHLDL TAPIPGASPE ETRQLLLEGS LRMKEGKDSK
601MDVYCFLFTD LLLVTKAVKK AERTRVIRPP LLVDKIVCRE LRDPGSFLLI YLNEFHSAVG
661AYTFQASGQA LCRGWVDTIY NAQNQLQQLR AQEPPGSQQP LQSLEEEEDE QEEEEEEEEE
721EEEGEDSGTS AASSPTIMRK SSGSPDSQHC ASDGSTETLA MVVVEPGDTL SSPEFDSGPF
781SSQSDETSLS TTASSATPTS ELLPLGPVDG RSCSMDSAYG TLSPTSLQDF VAPGPMAELV
841PRAPESPRVP SPPPSPRLRR RTPVQLLSCP PHLLKSKSEA SLLQLLAGAG THGTPSAPSR
901SLSELCLAVP APGIRTQGSP QEAGPSWDCR GAPSPGSGPG LVGCLAGEPA GSHRKRCGDL
961PSGASPRVQP EPPPGVSAQH RKLTLAQLYR IRTTLLLNST LTASEV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1006 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.