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PLEKHG4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHG4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLEKHG4 antibodies →
$725.00each
Catalog Number: LS-G124884-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHG4 (NM_001129727), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_015432
SwissProt
Q58EX7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERPLENGDE SPDSQGHATD WRFAVCSFRD AWEEEEPASQ MHVKDPGPPR PPAGATQDEE
61LQGSPLSRKF QLPPAADESG DAQRGTVESS SVLSEGPGPS GVESLLCPMS SHLSLAQGES
121DTPGVGLVGD PGPSRAMPSG LSPGALDSDP VGLGDPLSEI SKLLEAAPSG SGLPKPADCL
181LAQDLCWELL ASGMATLPGT RDVQGRAVLL LCAHSPAWLQ SECSSQELIR LLLYLRSIPR
241PEVQALGLTV LVDARICAPS SSLFSGLSQL QEAAPGAVYQ VLLVGSTLLK EVPSGLQLEQ
301LPSQSLLTHI PTAGLPTSLG GGLPYCHQAW LDFRRRLEAL LQNCQAACAL LQGAIESVKA
361VPQPMEPGEV GQLLQQTEVL MQQVLDSPWL AWLQCQGGRE LTWLKQEVPE VTLSPDYRTA
421MDKADELYDR VDGLLHQLTL QSNQRIQALE LVQTLEARES GLHQIEVWLQ QVGWPALEEA
481GEPSLDMLLQ AQGSFQELYQ VAQEQVRQGE KFLQPLTGWE AAELDPPGAR FLALRAQLTE
541FSRALAQRCQ RLADAERLFQ LFREALTWAE EGQRVLAELE QERPGVVLQQ LQLHWTRHPD
601LPPAHFRKMW ALATGLGSEA IRQECRWAWA RCQDTWLALD QKLEASLKLP PVGSTASLCV
661SQVPAAPAHP PLRKAYSFDR NLGQSLSEPA CHCHHAATIA ACRRPEAGGG ALPQASPTVP
721PPGSSDPRSL NRLQLVLAEM VATEREYVRA LEYTMENYFP ELDRPDVPQG LRGQRAHLFG
781NLEKLRDFHC HFFLRELEAC TRHPPRVAYA FLRHRVQFGM YALYSKNKPR SDALMSSYGH
841TFFKDKQQAL GDHLDLASYL LKPIQRMGKY ALLLQELARA CGGPTQELSA LREAQSLVHF
901QLRHGNDLLA MDAIQGCDVN LKEQGQLVRQ DEFVVRTGRH KSVRRIFLFE ELLLFSKPRH
961GPTGVDTFAY KRSFKMADLG LTECCGNSNL RFEIWFRRRK ARDTFVLQAS SLAIKQAWTA
1021DISHLLWRQA VHNKEVRMAE MVSMGVGNKA FRDIAPSEEA INDRTVNYVL KCREVRSRAS
1081IAVAPFDHDS LYLGASNSLP GDPASCSVLG SLNLHLYRDP ALLGLRCPLY PSFPEEAALE
1141AEAELGGQPS LTAEDSEISS QCPSASGSSG SDSSCVSGQA LGRGLEDLPC V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1191 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.