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PLEKHG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLEKHG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLEKHG1 antibodies →
$725.00each
Catalog Number: LS-G120809-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLEKHG1 (NM_001029884), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF41 | PLEKHG1 | KIAA1209
Origin Species
Human
NCBI GenBank
AB033035
SwissProt
Q9ULL1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
155.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELSDSDRPV SFGSTSSSAS SRDSHGSFGS RMTLVSNSHM GLFNQDKEVG AIKLELIPAR
61PFSSSELQRD NPATGQQNAD EGSERPPRAQ WRVDSNGAPK TIADSATSPK LLYVDRVVQE
121ILETERTYVQ DLKSIVEDYL DCIRDQTKLP LGTEERSALF GNIQDIYHFN SELLQDLENC
181ENDPVAIAEC FVSKSEEFHI YTQYCTNYPR SVAVLTECMR NKILAKFFRE RQETLKHSLP
241LGSYLLKPVQ RILKYHLLLH EIENHLDKDT EGYDVVLDAI DTMQRVAWHI NDMKRKHEHA
301VRLQEIQSLL TNWKGPDLTS YGELVLEGTF RIQRAKNERT LFLFDKLLLI TKKRDDTFTY
361KAHILCGNLM LVEVIPKEPL SFSVFHYKNP KLQHTVQAKS QQDKRLWVLH LKRLILENHA
421AKIPAKAKQA ILEMDAIHHP GFCYSPEGGT KALFGSKEGS APYRLRRKSE PSSRSHKVLK
481TSETAQDIQK VSREEGSPQL SSARPSPAQR NSQPSSSTMI SVLRAGGALR NIWTDHQIRQ
541ALFPSRRSPQ ENEDDEDDYQ MFVPSFSSSD LNSTRLCEDS TSSRPCSWHM GQMESTETSS
601SGHRIVRRAS SAGESNTCPP EIGTSDRTRE LQNSPKTEGQ EEMTPFGSSI ELTIDDIDHV
661YDNISYEDLK LMVAKREEAE STPSKSARDS VRPKSTPELA FTKRQAGHSK GSLYAQTDGT
721LSGGEASSQS THELQAVEEN IYDTIGLPDP PSLGFKCSSL KRAKRSTFLG LEADFVCCDS
781LRPFVSQDSL QLSEDEAPYH QATPDHGYLS LLYDSPSGNL SMPHKPVSDK LSEEVDEIWN
841DLENYIKKNE DKARDRLLAA FPVSKDDVPD RLHAESTPEL SRDVGRSVST LSLPESQALL
901TPVKSRAGRA SRANCPFEED LISKEGSFMS LNRLSLASEM PLMDNPYDLA NSGLSQTDPE
961NPDLGMEATD KTKSRVFMMA RQYSQKIKKA NQLLKVKSLE LEQPPASQHQ KSMHKDLAAI
1021LEEKKQGGPA IGARIAEYSQ LYDQIVFRES PLKIQKDGWA SPQESSLLRS VSPSQVHHGS
1081GDWLLHSTYS NGELADFCLP PEQDLRSRYP TFEINTKSTP RQLSAACSVP SLQTSDPLPG
1141SVQRCSVVVS QPNKENWCQD HLYNSLGRKG ISAKSQPYHR SQSSSSVLIN KSMDSINYPS
1201DVGKQQLLSL HRSSRCESHQ DLLPDIADSH QQGTEKLSDL TLQDSQKVVV VNRNLPLNAQ
1261IATQNYFSNF KETDGDEDDY VEIKSEEDES ELELSHNRRR KSDSKFVDAD FSDNVCSGNT
1321LHSLNSPRTP KKPVNSKLGL SPYLTPYNDS DKLNDYLWRG PSPNQQNIVQ SLREKFQCLS
1381SSSFA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1385 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.