Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PLD5 / Phospholipase D5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130978-100
Catalog Number: LS-G130978
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLD5 (NM_001195811), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Inactive choline phosphatase 5 | Inactive PLD 5 | Inactive phospholipase D5 | PLD5 | PLDC
- Origin Species
- Human
- SwissProt
- Q8N7P1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSQQKCIVIF ALVCCFAILV ALIFSAVDIM GEDEDGLSEK NCQNKCRIAL VENIPEGLNY61SENAPFHLSL FQGWMNLLNM AKKSVDIVSS HWDLNHTHPS ACQGQRLFEK LLQLTSQNIE121IKLVSDVTAD SKVLEALKLK GAEVTYMNMT AYNKGRLQSS FWIVDKQHVY IGSAGLDWQS181LGQMKELGVI FYNCSCLVLD LQRIFALYSS LKFKSRVPQT WSKRLYGVYD NEKKLQLQLN241ETKSQAFVSN SPKLFCPKNR SFDIDAIYSV IDDAKQYVYI AVMDYLPISS TSTKRTYWPD301LDAKIREALV LRSVRVRLLL SFWKETDPLT FNFISSLKAI CTEIANCSLK VKFFDLEREN361ACATKEQKNH TFPRLNRNKY MVTDGAAYIG NFDWVGNDFT QNAGTGLVIN QADVRNNRSI421IKQLKDVFER DWYSPYAKTL QPTKQPNCSS LFKLKPLSNK TATDDTGGKD PRNVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
474 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.