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PLCL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLCL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113407-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLCL2 (NM_015184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Phospholipase C-like 2 | PLC-L(2) | PLC-L2 | PLCE2 | KIAA1092 | Phospholipase C | epsilon 2 | Phospholipase C-epsilon-2 | Phospholipase C-L2 | PLC-epsilon-2 | PLCL2
Origin Species
Human
NCBI GenBank
NM_015184
SwissProt
Q9UPR0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTEKKISSA SDCINSMVEG SELKKVRSNS RIYHRYFLLD ADMQSLRWEP SKKDSEKAKI
61DIKSIKEVRT GKNTDIFRSN GISDRISEDC AFSVIYGENY ESLDLVANSA DVANIWVTGL
121RYLISYGKHT LDMLESSQDN MRTSWVSQMF SEIDVDNLGH ITLCNAVQCI RNLNPGLKTS
181KIELKFKELH KSKDKAGTEV TKEEFIEVFH ELCTRPEIYF LLVQFSSNKE FLDTKDLMMF
241LEAEQGVAHI NEEISLEIIH KYEPSKEGQE KGWLSIDGFT NYLMSPDCYI FDPEHKKVCQ
301DMKQPLSHYF INSSHNTYLI EDQFRGPSDI TGYIRALKMG CRSVELDVWD GPDNEPVIYT
361GHTMTSQIVF RSVIDIINKY AFFASEYPLI LCLENHCSIK QQKVMVQHMK KLLGDKLYTT
421SPNVEESYLP SPDVLKGKIL IKAKKLSSNC SGVEGDVTDE DEGAEMSQRM GKENMEQPNN
481VPVKRFQLCK ELSELVSICK SVQFKEFQMS FQVQKYWEVC SFNEVLASKY ANENPGDFVN
541YNKRFLARVF PSPMRIDSSN MNPQDFWKCG CQIVAMNFQT PGLMMDLNIG WFRQNGNCGY
601VLRPAIMREE VSFFSVNTKD SVPGVSPQLL HIKIISGQNF PKPKGSGAKG DVVDPYVYVE
661IHGIPADCAE QRTKTVHQNG DAHIFDESFE FQINLPELAM VRFVVLDDDY IGDEFIGQYT
721IPFECLQTGY RHVPLQSLTG EVLAHASLFV HVAITNRRGG GKPRKRGLSV RKGKKSREYA
781SLRTLWIKTV DEVFKNAQPP IRDATDLREN MQNAVVSFKE LCGLSSVANL MQCMLAVSPR
841FLGPDNTPLV VLNLSEQYPT MELQGIVPEV LKKIVTTYDM MIQSLKALIE NADAVYEKIV
901HCQKAAMEFH EHLHSIGTKE GLKERKLQKA VESFTWNITI LKGQADLLKY AKNETLENLK
961QIHFAAVSCG LNKPGTENAD VQKPRRSLEV IPEKANDETG E
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1001 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.