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PLCL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLCL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129157-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLCL1 (NM_006226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Phospholipase C | epsilon | PLCL1 | PRIP | Phospholipase c | PLDL1 | Phospholipase C-like 1 | PLC-L | PLC-L1 | PLCE | PLCL
Origin Species
Human
NCBI GenBank
NM_006226

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
122.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEGAAGRED PAPPDAAGGE DDPRVGPDAA GDCVTAASGG RMRDRRSGVA LPGAAGTPAD
61SEAGLLEAAR ATPRRSSIIK DPSNQKCGGR KKTVSFSSMP SEKKISSAND CISFMQAGCE
121LKKVRPNSRI YNRFFTLDTD LQALRWEPSK KDLEKAKLDI SAIKEIRLGK NTETFRNNGL
181ADQICEDCAF SILHGENYES LDLVANSADV ANIWVSGLRY LVSRSKQPLD FMEGNQNTPR
241FMWLKTVFEA ADVDGNGIML EDTSVELIKQ LNPTLKEAKI RLKFKEIQKS KEKLTTRVTE
301EEFCEAFCEL CTRPEVYFLL VQISKNKEYL DANDLMLFLE AEQGVTHITE DICLDIIRRY
361ELSEEGRQKG FLAIDGFTQY LLSSECDIFD PEQKKVAQDM TQPLSHYYIN ASHNTYLIED
421QFRGPADING YIRALKMGCR SVELDVSDGS DNEPILCNRN NMTTHVSFRS VIEVINKFAF
481VASEYPLILC LGNHCSLPQQ KVMAQQMKKV FGNKLYTEAP LPSESYLPSP EKLKRMIIVK
541GKKLPSDPDV LEGEVTDEDE EAEMSRRMSV DYNGEQKQIR LCRELSDLVS ICKSVQYRDF
601ELSMKSQNYW EMCSFSETEA SRIANEYPED FVNYNKKFLS RIYPSAMRID SSNLNPQDFW
661NCGCQIVAMN FQTPGPMMDL HTGWFLQNGG CGYVLRPSIM RDEVSYFSAN TKGILPGVSP
721LALHIKIISG QNFPKPKGAC AKGDVIDPYV CIEIHGIPAD CSEQRTKTVQ QNSDNPIFDE
781TFEFQVNLPE LAMIRFVVLD DDYIGDEFIG QYTIPFECLQ PGYRHVPLRS FVGDIMEHVT
841LFVHIAITNR SGGGKAQKRS LSVRMGKKVR EYTMLRNIGL KTIDDIFKIA VHPLREAIDM
901RENMQNAIVS IKELCGLPPI ASLKQCLLTL SSRLITSDNT PSVSLVMKDS FPYLEPLGAI
961PDVQKKMLTA YDLMIQESRF LIEMADTVQE KIVQCQKAGM EFHEELHNLG AKEGLKGRKL
1021NKATESFAWN ITVLKGQGDL LKNAKNEAIE NMKQIQLACL SCGLSKAPSS SAEAKSKRSL
1081EAIEEKESSE ENGKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1095 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.