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PLCH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLCH2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLCH2 antibodies →
$725.00each
Catalog Number: LS-G113808-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLCH2 (NM_014638), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Phospholipase C-eta-2 | Phospholipase C-eta2 | Phospholipase C-like 4 | Phospholipase C-like protein 4 | Phospholipase C | eta 2 | PLCH2 | PLC-eta2 | RP3-395M20.1 | KIAA0450 | PLC-L4 | PLCeta2 | PLCL4
Origin Species
Human
NCBI GenBank
AB007919
SwissProt
O75038

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
154.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGPWPSPDS RTKGTVAWLA EVLLWVGGSV VLSSEWQLGP LVERCMGAMQ EGMQMVKLRG
61GSKGLVRFYY LDEHRSCIRW RPSRKNEKAK ISIDSIQEVS EGRQSEVFQR YPDGSFDPNC
121CFSIYHGSHR ESLDLVSTSS EVARTWVTGL RYLMAGISDE DSLARRQRTR DQWLKQTFDE
181ADKNGDGSLS IGEVLQLLHK LNVNLPRQRV KQMFREADTD DHQGTLGFEE FCAFYKMMST
241RRDLYLLMLT YSNHKDHLDA ASLQRFLQVE QKMAGVTLES CQDIIEQFEP CPENKSKGLL
301GIDGFTNYTR SPAGDIFNPE HHHVHQDMTQ PLSHYFITSS HNTYLVGDQL MSQSRVDMYA
361WVLQAGCRCV EVDCWDGPDG EPIVHHGYTL TSKILFKDVI ETINKYAFIK NEYPVILSIE
421NHCSVIQQKK MAQYLTDILG DKLDLSSVSS EDATTLPSPQ MLKGKILVKG KKLPANISED
481AEEGEVSDED SADEIDDDCK LLNGDASTNR KRVENTAKRK LDSLIKESKI RDCEDPNNFS
541VSTLSPSGKL GRKSKAEEDV ESGEDAGASR RNGRLVVGSF SRRKKKGSKL KKAASVEEGD
601EGQDSPGGQS RGATRQKKTM KLSRALSDLV KYTKSVATHD IEMEAASSWQ VSSFSETKAH
661QILQQKPAQY LRFNQQQLSR IYPSSYRVDS SNYNPQPFWN AGCQMVALNY QSEGRMLQLN
721RAKFSANGGC GYVLKPGCMC QGVFNPNSED PLPGQLKKQL VLRIISGQQL PKPRDSMLGD
781RGEIIDPFVE VEIIGLPVDC SREQTRVVDD NGFNPTWEET LVFMVHMPEI ALVRFLVWDH
841DPIGRDFIGQ RTLAFSSMMP GYRHVYLEGM EEASIFVHVA VSDISGKVKQ ALGLKGLFLR
901GPKPGSLDSH AAGRPPARPS VSQRILRRTA SAPTKSQKPG RRGFPELVLG TRDTGSKGVA
961DDVVPPGPGP APEAPAQEGP GSGSPRDTRP LSTQRPLPPL CSLETIAEEP APGPGPPPPA
1021AVPTSSSQGR PPYPTGPGAN VASPLEDTEE PRDSRPRPCN GEGAGGAYER APGSQTDGRS
1081QPRTLGHLPV IRRVKSEGQV PTEPLGGWRP LAAPFPAPAV YSDATGSDPL WQRLEPCGHR
1141DSVSSSSSMS SSDTVIDLSL PSLGLGRSRE NLAGAHMGRL PPRPHSASAA RPDLPPVTKS
1201KSNPNLRATG QRPPIPDELQ PRSLAPRMAG LPFRPPWGCL SLVGVQDCPV AAKSKSLGDL
1261TADDFAPSFE GGSRRLSHSL GLPGGTRRVS GPGVRRDTLT EQLRWLTVFQ QAGDITSPTS
1321LGPAGEGVAG GPGFVRRSSS RSHSRVRAIA SRARQAQERQ QRLQGLGRQG PPEEERGTPE
1381GACSVGHEGS VDAPAPSKGA LGPASAAAEN LVLLRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1416 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.