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PLCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLCH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLCH1 antibodies →
$725.00each
Catalog Number: LS-G125168-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Phospholipase c eta 1(PLCH1) (NM_001130960), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1069 | Phospholipase C-like 3 | Phospholipase C-like protein 3 | Phospholipase C-eta-1 | PLC eta 1 | PLC-eta-1 | PLC-L3 | PLCeta1 | PLCL3 | PLCH1 | Phospholipase C-eta1a | Phospholipase C-eta1b | Phospholipase C | eta 1
Origin Species
Human
NCBI GenBank
AB028992
SwissProt
Q4KWH8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
189 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADLEVYKNL SPEKVERCMS VMQSGTQMIK LKRGTKGLVR LFYLDEHRTR LRWRPSRKSE
61KAKILIDSIY KVTEGRQSEI FHRQAEGNFD PSCCFTIYHG NHMESLDLIT SNPEEARTWI
121TGLKYLMAGI SDEDSLAKRQ RTHDQWVKQT FEEADKNGDG LLNIEEIHQL MHKLNVNLPR
181RKVRQMFQEA DTDENQGTLT FEEFCVFYKM MSLRRDLYLL LLSYSDKKDH LTVEELAQFL
241KVEQKMNNVT TDYCLDIIKK FEVSEENKVK NVLGIEGFTN FMRSPACDIF NPLHHEVYQD
301MDQPLCNYYI ASSHNTYLTG DQLLSQSKVD MYARVLQEGC RCVEVDCWDG PDGEPVVHHG
361YTLTSKILFR DVVETINKHA FVKNEFPVIL SIENHCSIQQ QRKIAQYLKG IFGDKLDLSS
421VDTGECKQLP SPQSLKGKIL VKGKKLPYHL GDDAEEGEVS DEDSADEIED ECKFKLHYSN
481GTTEHQVESF IRKKLESLLK ESQIRDKEDP DSFTVRALLK ATHEGLNAHL KQSPDVKESG
541KKSHGRSLMT NFGKHKKTTK SRSKSYSTDD EEDTQQSTGK EGGQLYRLGR RRKTMKLCRE
601LSDLVVYTNS VAAQDIVDDG TTGNVLSFSE TRAHQVVQQK SEQFMIYNQK QLTRIYPSAY
661RIDSSNFNPL PYWNAGCQLV ALNYQSEGRM MQLNRAKFKA NGNCGYVLKP QQMCKGTFNP
721FSGDPLPANP KKQLILKVIS GQQLPKPPDS MFGDRGEIID PFVEVEIIGL PVDCCKDQTR
781VVDDNGFNPV WEETLTFTVH MPEIALVRFL VWDHDPIGRD FVGQRTVTFS SLVPGYRHVY
841LEGLTEASIF VHITINEIYG KWSPLILNPS YTILHFLGAT KNRQLQGLKG LFNKNPRHSS
901SENNSHYVRK RSIGDRILRR TASAPAKGRK KSKMGFQEMV EIKDSVSEAT RDQDGVLRRT
961TRSLQARPVS MPVDRNLLGA LSLPVSETAK DIEGKENSLA EDKDGRRKGK ASIKDPHFLN
1021FNKKLSSSSS ALLHKDTSQG DTIVSTAHMS VTGEQLGMSS PRGGRTTSNA TSNCQENPCP
1081SKSLSPKQHL APDPVVNPTQ DLHGVKIKEK GNPEDFVEGK SILSGSVLSH SNLEIKNLEG
1141NRGKGRAATS FSLSDVSMLC SDIPDLHSTA ILQESVISHL IDNVTLTNEN EPGSSISALI
1201GQFDETNNQA LTVVSHLHNT SVMSGHCPLP SLGLKMPIKH GFCKGKSKSS FLCSSPELIA
1261LSSSETTKHA TNTVYETTCT PISKTKPDDD LSSKAKTAAL ESNLPGSPNT SRGWLPKSPT
1321KGEDWETLKS CSPASSPDLT LEDVIADPTL CFNSGESSLV EIDGESENLS LTTCEYRREG
1381TSQLASPLKL KYNQGVVEHF QRGLRNGYCK ETLRPSVPEI FNNIQDVKTQ SISYLAYQGA
1441GFVHNHFSDS DAKMFQTCVP QQSSAQDMHV PVPKQLAHLP LPALKLPSPC KSKSLGDLTS
1501EDIACNFESK YQCISKSFVT TGIRDKKGVT VKTKSLEPID ALTEQLRKLV SFDQEDNCQV
1561LYSKQDANQL PRALVRKLSS RSQSRVRNIA SRAKEKQEAN KQKVPNPSNG AGVVLRNKPS
1621APTPAVNRHS TGSYIAGYLK NTKGGGLEGR GIPEGACTAL HYGHVDQFCS DNSVLQTEPS
1681SDDKPEIYFL LRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1693 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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