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PLCB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLCB4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLCB4 antibodies →
$725.00each
Catalog Number: LS-G130403-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Phospholipase C beta 4(PLCB4) (NM_001172646), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARCND2 | Phosphoinositidase C | Phospholipase C-beta-4 | PI-PLC | Inositoltrisphosphohydrolase | PLC-beta-4 | PLCB4 | Phospholipase C | beta 4
Origin Species
Human
NCBI GenBank
NM_000933

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKPYEFNWQ KEVPSFLQEG TVFDRYEEES FVFEPNCLFK VDEFGFFLTW RSEGKEGQVL
61ECSLINSIRS GAIPKDPKIL AALEAVGKSE NDLEGRIVCV CSGTDLVNIS FTYMVAENPE
121VTKQWVEGLR SIIHNFRANN VSPMTCLKKH WMKLAFMTNT NGKIPVRSIT RTFASGKTEK
181VIFQALKELG LPSGKNDEIE PTAFSYEKFY ELTQKICPRT DIEDLFKKIN GDKTDYLTVD
241QLVSFLNEHQ RDPRLNEILF PFYDAKRAMQ IIEMYEPDED LKKKGLISSD GFCRYLMSDE
301NAPVFLDRLE LYQEMDHPLA HYFISSSHNT YLTGRQFGGK SSVEMYRQVL LAGCRCVELD
361CWDGKGEDQE PIITHGKAMC TDILFKDVIQ AIKETAFVTS EYPVILSFEN HCSKYQQYKM
421SKYCEDLFGD LLLKQALESH PLEPGRALPS PNDLKRKILI KNKRLKPEVE KKQLEALRSM
481MEAGESASPA NILEDDNEEE IESADQEEEA HPEFKFGNEL SADDLGHKEA VANSVKKASD
541DLEHENNKKG LVTVEDEQAW MASYKYVGAT TNIHPYLSTM INYAQPVKFQ GFHVAEERNI
601HYNMSSFNES VGLGYLKTHA IEFVNYNKRQ MSRIYPKGGR VDSSNYMPQI FWNAGCQMVS
661LNYQTPDLAM QLNQGKFEYN GSCGYLLKPD FMRRPDRTFD PFSETPVDGV IAATCSVQVI
721SGQFLSDKKI GTYVEVDMYG LPTDTIRKEF RTRMVMNNGL NPVYNEESFV FRKVILPDLA
781VLRIAVYDDN NKLIGQRILP LDGLQAGYRH ISLRNEGNKP LSLPTIFCNI VLKTYVPDGF
841GDIVDALSDP KKFLSITEKR ADQMRAMGIE TSDIADVPSD TSKNDKKGKA NTAKANVTPQ
901SSSELRPTTT AALASGVEAK KGIELIPQVR IEDLKQMKAY LKHLKKQQKE LNSLKKKHAK
961EHSTMQKLHC TQVDKIVAQY DKEKSTHEKI LEKAMKKKGG SNCLEMKKET EIKIQTLTSD
1021HKSKVKEIVA QHTKEWSEMI NTHSAEEQEI RDLHLSQQCE LLKKLLINAH EQQTQQLKLS
1081HDRESKEMRA HQAKISMENS KAISQDKSIK NKAERERRVR ELNSSNTKKF LEERKRLAMK
1141QSKEMDQLKK VQLEHLEFLE KQNEQAKEMQ QMVKLEAEMD RRPATVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1187 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.