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PLBD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLBD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128229-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLBD2 (NM_001159727), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
76 kDa protein | Lamina ancestor homolog 2 | PLB homolog 2 | PLBD2 | LAMA-like protein 2 | p76
Origin Species
Human
NCBI GenBank
NM_001159727

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.88 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVGQMYCYPG SHLARALTRA LALALVLALL VGPFLSGLAG AIPAPGGRWA RDGQVPPASR
61SRSVLLDVSA GQLLMVDGRH PDAVAWANLT NAIRETGWAF LELGTSGQYN DSLQAYAAGV
121VEAAVSEELI YMHWMNTVVN YCGPFEYEVG YCERLKSFLE ANLEWMQEEM ESNPDSPYWH
181QVRLTLLQLK GLEDSYEGRV SFPAGKFTIK PLGFLLLQLS GDLEDLELAL NKTKIKPSLG
241SGSCSALIKL LPGQSDLLVA HNTWNNYQHM LRVIKKYWLQ FREGPWGDYP LVPGNKLVFS
301SYPGTIFSCD DFYILGSGLV TLETTIGNKN PALWKYVRPR GCVLEWVRNI VANRLASDGA
361TWADIFKRFN SGTGMVVVAD KTSELYQKTY WASYNIPSFE TVFNASGLQA LVAQYGDWFS
421YDGSPRAQIF RRNQSLVQDM DSMVRLMRYN DFLHDPLSLC KACNPQPNGE NAISARSDLN
481PANGSYPFQA LRQRSHGGID VKVTSMSLAR ILSLLAASGP TWDQVPPFQW STSPFSGLLH
541MGQPDLWKFA PVKVSWD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

557 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.