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PLBD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLBD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109637-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLBD1 (NM_024829), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Phospholipase B-like 1 | PLBD1 | LAMA-like protein 1 | Lamina ancestor homolog 1 | PLB homolog 1
Origin Species
Human
NCBI GenBank
NM_024829
SwissProt
Q6P4A8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTRGGPGGRP GLPQPPPLLL LLLLPLLLVT AEPPKPAGVY YATAYWMPAE KTVQVKNVMD
61KNGDAYGFYN NSVKTTGWGI LEIRAGYGSQ TLSNEIIMFV AGFLEGYLTA PHMNDHYTNL
121YPQLITKPSI MDKVQDFMEK QDKWTRKNIK EYKTDSFWRH TGYVMAQIDG LYVGAKKRAI
181LEGTKPMTLF QIQFLNSVGD LLDLIPSLSP TKNGSLKVFK RWDMGHCSAL IKVLPGFENI
241LFAHSSWYTY AAMLRIYKHW DFNIIDKDTS SSRLSFSSYP GFLESLDDFY ILSSGLILLQ
301TTNSVFNKTL LKQVIPETLL SWQRVRVANM MADSGKRWAD IFSKYNSGTY NNQYMVLDLK
361KVKLNHSLDK GTLYIVEQIP TYVEYSEQTD VLRKGYWPSY NVPFHEKIYN WSGYPLLVQK
421LGLDYSYDLA PRAKIFRRDQ GKVTDTASMK YIMRYNNYKK DPYSRGDPCN TICCREDLNS
481PNPSPGGCYD TKVADIYLAS QYTSYAISGP TVQGGLPVFR WDRFNKTLHQ GMAEVYNFDF
541ITMKPILKLD IK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

552 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.