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PLAGL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLAGL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117846-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLAGL2 (NM_002657), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein PLAGL2 | ZNF900 | C2H2-type zinc finger protein | KIAA0198 | PLAGL2
Origin Species
Human
NCBI GenBank
NM_002657
SwissProt
Q9UPG8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTFFTSVPP WIQDAKQEEE VGWKLVPRPR GREAESQVKC QCEISGTPFS NGEKLRPHSL
61PQPEQRPYSC PQLHCGKAFA SKYKLYRHMA THSAQKPHQC MYCDKMFHRK DHLRNHLQTH
121DPNKEALHCS ECGKNYNTKL GYRRHLAMHA ASSGDLSCKV CLQTFESTQA LLEHLKAHSR
181RVAGGAKEKK HPCDHCDRRF YTRKDVRRHL VVHTGRKDFL CQYCAQRFGR KDHLTRHVKK
241SHSQELLKIK TEPVDMLGLL SCSSTVSVKE ELSPVLCMAS RDVMGTKAFP GMLPMGMYGA
301HIPTMPSTGV PHSLVHNTLP MGMSYPLESS PISSPAQLPP KYQLGSTSYL PDKLPKVEVD
361SFLAELPGSL SLSSAEPQPA SPQPAAAAAL LDEALLAKSP ANLSEALCAA NVDFSHLLGF
421LPLNLPPCNP PGATGGLVMG YSQAEAQPLL TTLQAQPQDS PGAGGPLNFG PLHSLPPVFT
481SGLSSTTLPR FHQAFQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.