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PLAG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLAG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117844-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLAG1 (NM_002655), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COL1A2/PLAG1 fusion | Pleiomorphic adenoma gene 1 | PLAG1 | PSA | SGPA | ZNF912 | Zinc finger protein PLAG1
Origin Species
Human
NCBI GenBank
NM_002655
SwissProt
Q6DJT9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATVIPGDLS EVRDTQKVPS GKRKRGETKP RKNFPCQLCD KAFNSVEKLK VHSYSHTGER
61PYKCIQQDCT KAFVSKYKLQ RHMATHSPEK THMCNYCEKM FHRKDHLKNH LHTHDPNKET
121FKCEECGKNY NTKLGFKRHL ALHAATSGDL TCKVCLQTFE STGVLLEHLK SHAGKSSGGV
181KEKKHQCEHC DRRFYTRKDV RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL
241LKVKTEPVDF LDPFTCNVSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS
301SGSAHQMITT LPLGMTCPID MDTVHPSHHL SFKYPFSSTS YAISIPEKEQ PLKGEIESYL
361MELQGGVPSS SQDSQASSSS KLGLDPQIGS LDDGAGDLSL SKSSISISDP LNTPALDFSQ
421LFNFIPLNGP PYNPLSVGSL GMSYSQEEAH SSVSQLPPQT QDLQDPANTI GLGSLHSLSA
481AFTSSLSTST TLPRFHQAFQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

500 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.