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PLA2G4E Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLA2G4E Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119647-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLA2G4E (NM_001080490), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPLA2-epsilon | Phospholipase A2 group IVE | PLA2G4E | Phospholipase A2 | group IVE
Origin Species
Human
SwissProt
Q3MJ16

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MRAKCITEPV GIELAPCLQA PSVPETDLKG VVQARGGGAS VLEKPREGFK RAEQVPVSQT
61DCFVSLWLPT ASQKKLRTRT ISNCPNPEWN ESFNFQIQSR VKNVLELSVC DEDTVTPDDH
121LLTVLYDLTK LCFRKKTHVK FPLNPQGMEE LEVEFLLEES PSPPETLVTN GVLVSRQVSC
181LEVHAQSRRR RKREKMKDLL VMVNESFENT QRVRPCLEPC CPTSACFQTA ACFHYPKYFQ
241SQVHVEVPKS HWSCGLCCRS RKKGPISQPL DCLSDGQVMT LPVGESYELH MKSTPCPETL
301DVRLGFSLCP AELEFLQKRK VVVAKALKQV LQLEEDLQED EVPLIAIMAT GGGTRSMTSM
361YGHLLGLQKL NLLDCASYIT GLSGATWTMA TLYRDPDWSS KNLEPAIFEA RRHVVKDKLP
421SLFPDQLRKF QEELRQRSQE GYRVTFTDFW GLLIETCLGD ERNECKLSDQ RAALSCGQNP
481LPIYLTINVK DDVSNQDFRE WFEFSPYEVG LQKYGAFIPS ELFGSEFFMG RLVKRIPESR
541ICYMLGLWSS IFSLNLLDAW NLSHTSEEFF HRWTREKVQD IEDEPILPEI PKCDANILET
601TVVIPGSWLS NSFREILTHR SFVSEFHNFL SGLQLHTNYL QNGQFSRWKD TVLDGFPNQL
661TESANHLCLL DTAFFVNSSY PPLLRPERKA DLIIHLNYCA GSQTKPLKQT CEYCTVQNIP
721FPKYELPDEN ENLKECYLME NPQEPDAPIV TFFPLINDTF RKYKAPGVER SPEELEQGQV
781DIYGPKTPYA TKELTYTEAT FDKLVKLSEY NILNNKDTLL QALRLAVEKK KRLKGQCPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

839 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.