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PLA2G4D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PLA2G4D Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PLA2G4D antibodies →
$725.00each
Catalog Number: LS-G104769-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PLA2G4D (NM_178034), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CPLA2delta | Phospholipase A2 group IVD | PLA2G4D | CPLA2-delta
Origin Species
Human
SwissProt
Q86XP0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESLSPGGPP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQ LSTAPGMKFK
61TKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTE DDICFKVLYD ISEVLPGKLL
121RKTFSQSPQG EEELDVEFLM EETSDRPENL ITNKVIVARE LSCLDVHLDS TGSTAVVADQ
181DKLELELVLK GSYEDTQTSF LGTASAFRFH YMAALETELS GRLRSSRSNG WNGDNSAGYL
241TVPLRPLTIG KEVTMDVPAP NAPGVRLQLK AEGCPEELAV HLGFNLCAEE QAFLSRRKQV
301VAKALKQALQ LDRDLQEDEV PVVGIMATGG GARAMTSLYG HLLALQKLGL LDCVTYFSGI
361SGSTWTMAHL YGDPEWSQRD LEGPIRYARE HLAKSKLEVF SPERLASYRR ELELRAEQGH
421PTTFVDLWAL VLESMLHGQV MDQKLSGQRA ALERGQNPLP LYLSLNVKEN NLETLDFKEW
481VEFSPYEVGF LKYGAFVPPE LFGSEFFMGR LMRRIPEPRI CFLEAIWSNI FSLNLLDAWY
541DLTSSGESWK QHIKDKTRSL EKEPLTTSGT SSRLEASWLQ PGTALAQAFK GFLTGRPLHQ
601RSPNFLQGLQ LHQDYCSHKD FSTWADYQLD SMPSQLTPKE PRLCLVDAAY FINTSSPSMF
661RPGRRLDLIL SFDYSLSAPF EALQQTELYC RARGLPFPRV EPSPQDQHQP RECHLFSDPA
721CPEAPILLHF PLVNASFKDH SAPGVQRSPA ELQGGQVDLT GATCPYTLSN MTYKEEDFER
781LLRLSDYNVQ TSQGAILQAL RTALKHRTLE ARPPRAQT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

818 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.