Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PKP4 / Plakophilin 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117193-100
Catalog Number: LS-G117193
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plakophilin 4(PKP4) (NM_003628), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PKP4 | p0071 | Plakophilin 4 | Catenin 4 | Plakophilin-4
- Origin Species
- Human
- NCBI GenBank
- NM_003628
- SwissProt
- Q99569
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 131.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPAPEQASLV EEGQPQTRQE AASTGPGMEP ETTATTILAS VKEQELQFQR LTRELEVERQ61IVASQLERCR LGAESPSIAS TSSTEKSFPW RSTDVPNTGV SKPRVSDAVQ PNNYLIRTEP121EQGTLYSPEQ TSLHESEGSL GNSRSSTQMN SYSDSGYQEA GSFHNSQNVS KADNRQQHSF181IGSTNNHVVR NSRAEGQTLV QPSVANRAMR RVSSVPSRAQ SPSYVISTGV SPSRGSLRTS241LGSGFGSPSV TDPRPLNPSA YSSTTLPAAR AASPYSQRPA SPTAIRRIGS VTSRQTSNPN301GPTPQYQTTA RVGSPLTLTD AQTRVASPSQ GQVGSSSPKR SGMTAVPQHL GPSLQRTVHD361MEQFGQQQYD IYERMVPPRP DSLTGLRSSY ASQHSQLGQD LRSAVSPDLH ITPIYEGRTY421YSPVYRSPNH GTVELQGSQT ALYRTGSVGI GNLQRTSSQR STLTYQRNNY ALNTTATYAE481PYRPIQYRVQ ECNYNRLQHA VPADDGTTRS PSIDSIQKDP REFAWRDPEL PEVIHMLQHQ541FPSVQANAAA YLQHLCFGDN KVKMEVCRLG GIKHLVDLLD HRVLEVQKNA CGALRNLVFG601KSTDENKIAM KNVGGIPALL RLLRKSIDAE VRELVTGVLW NLSSCDAVKM TIIRDALSTL661TNTVIVPHSG WNNSSFDDDH KIKFQTSLVL RNTTGCLRNL SSAGEEARKQ MRSCEGLVDS721LLYVIHTCVN TSDYDSKTVE NCVCTLRNLS YRLELEVPQA RLLGLNELDD LLGKESPSKD781SEPSCWGKKK KKKKRTPQED QWDGVGPIPG LSKSPKGVEM LWHPSVVKPY LTLLAESSNP841ATLEGSAGSL QNLSAGNWKF AAYIRAAVRK EKGLPILVEL LRMDNDRVVS SVATALRNMA901LDVRNKELIG KYAMRDLVNR LPGGNGPSVL SDETMAAICC ALHEVTSKNM ENAKALADSG961GIEKLVNITK GRGDRSSLKV VKAAAQVLNT LWQYRDLRSI YKKDGWNQNH FITPVSTLER1021DRFKSHPSLS TTNQQMSPII QSVGSTSSSP ALLGIRDPRS EYDRTQPPMQ YYNSQGDATH1081KGLYPGSSKP SPIYISSYSS PAREQNRRLQ HQQLYYSQDD SNRKNFDAYR LYLQSPHSYE1141DPYFDDRVHF PASTDYSTQY GLKSTTNYVD FYSTKRPSYR AEQYPGSPDS WVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1192 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.