Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PKP3 / Plakophilin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114798-100
Catalog Number: LS-G114798
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plakophilin 3(PKP3) (NM_007183), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PKP3 | Plakophilin 3 | Plakophilin-3 | Plakophilin 3b
- Origin Species
- Human
- NCBI GenBank
- NM_007183
- SwissProt
- Q9Y446
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQDGNFLLSA LQPEAGVCSL ALPSDLQLDR RGAEGPEAER LRAARVQEQV RARLLQLGQQ61PRHNGAAEPE PEAETARGTS RGQYHTLQAG FSSRSQGLSG DKTSGFRPIA KPAYSPASWS121SRSAVDLSCS RRLSSAHNGG SAFGAAGYGG AQPTPPMPTR PVSFHERGGV GSRADYDTLS181LRSLRLGPGG LDDRYSLVSE QLEPAATSTY RAFAYERQAS SSSSRAGGLD WPEATEVSPS241RTIRAPAVRT LQRFQSSHRS RGVGGAVPGA VLEPVARAPS VRSLSLSLAD SGHLPDVHGF301NSYGSHRTLQ RLSSGFDDID LPSAVKYLMA SDPNLQVLGA AYIQHKCYSD AAAKKQARSL361QAVPRLVKLF NHANQEVQRH ATGAMRNLIY DNADNKLALV EENGIFELLR TLREQDDELR421KNVTGILWNL SSSDHLKDRL ARDTLEQLTD LVLSPLSGAG GPPLIQQNAS EAEIFYNATG481FLRNLSSASQ ATRQKMRECH GLVDALVTSI NHALDAGKCE DKSVENAVCV LRNLSYRLYD541EMPPSALQRL EGRGRRDLAG APPGEVVGCF TPQSRRLREL PLAADALTFA EVSKDPKGLE601WLWSPQIVGL YNRLLQRCEL NRHTTEAAAG ALQNITAGDR RWAGVLSRLA LEQERILNPL661LDRVRTADHH QLRSLTGLIR NLSRNARNKD EMSTKVVSHL IEKLPGSVGE KSPPAEVLVN721IIAVLNNLVV ASPIAARDLL YFDGLRKLIF IKKKRDSPDS EKSSRAASSL LANLWQYNKL781HRDFRAKGYR KEDFLGPTRT RPLEQKLISE EDLAANDILD YKDDDDKV
828 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.