Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PKP1 / Plakophilin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G122350-100
Catalog Number: LS-G122350
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Plakophilin 1(PKP1) (NM_001005337), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- B6P | Band 6 protein | PKP1 | Plakophilin 1 | Plakophilin-1 | Plakophilin
- Origin Species
- Human
- NCBI GenBank
- NM_000299
- SwissProt
- Q13835
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNHSPLKTAL AYECFQDQDN STLALPSDQK MKTGTSGRQR VQEQVMMTVK RQKSKSSQSS61TLSHSNRGSM YDGLADNYNY GTTSRSSYYS KFQAGNGSWG YPIYNGTLKR EPDNRRFSSY121SQMENWSRHY PRGSCNTTGA GSDICFMQKI KASRSEPDLY CDPRGTLRKG TLGSKGQKTT181QNRYSFYSTC SGQKAIKKCP VRPPSCASKQ DPVYIPPISC NKDLSFGHSR ASSKICSEDI241ECSGLTIPKA VQYLSSQDEK YQAIGAYYIQ HTCFQDESAK QQVYQLGGIC KLVDLLRSPN301QNVQQAAAGA LRNLVFRSTT NKLETRRQNG IREAVSLLRR TGNAEIQKQL TGLLWNLSST361DELKEELIAD ALPVLADRVI IPFSGWCDGN SNMSREVVDP EVFFNATGCL RNLSSADAGR421QTMRNYSGLI DSLMAYVQNC VAASRCDDKS VENCMCVLHN LSYRLDAEVP TRYRQLEYNA481RNAYTEKSST GCFSNKSDKM MNNNYDCPLP EEETNPKGSG WLYHSDAIRT YLNLMGKSKK541DATLEACAGA LQNLTASKGL MSSGMSQLIG LKEKGLPQIA RLLQSGNSDV VRSGASLLSN601MSRHPLLHRV MGNQVFPEVT RLLTSHTGNT SNSEDILSSA CYTVRNLMAS QPQLAKQYFS661SSMLNNIINL CRSSASPKAA EAARLLLSDM WSSKELQGVL RQQGFDRNML GTLAGANSLR721NFTSRFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
726 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.