Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PITX2 / RGS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99008-100
Catalog Number: LS-G99008
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PITX2 (NM_000325), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- All1-responsive gene 1 | ARP1 | Brx1 | Homeobox protein PITX2 | IGDS2 | IRID2 | Iridogoniodysgenesis type 2 | IGDS | Pituitary homeobox 2 | PTX2 | ALL1-responsive protein ARP1 | RGS | Solurshin | Otlx2 | Paired-like homeodomain 2 | PITX2 | RIEG | RIEG1 | RS | IDG2 | IHG2
- NCBI GenBank
- NM_000325
- SwissProt
- Q99697
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNCMKGPLHL EHRAAGTKLS AVSSSSCHHP QPLAMASVLA PGQPRSLDSS KHRLEVHTIS61DTSSPEAAEK DKSQQGKNED VGAEDPSKKK RQRRQRTHFT SQQLQELEAT FQRNRYPDMS121TREEIAVWTN LTEARVRVWF KNRRAKWRKR ERNQQAELCK NGFGPQFNGL MQPYDDMYPG181YSYNNWAAKG LTSASLSTKS FPFFNSMNVN PLSSQSMFSP PNSISSMSMS SSMVPSAVTG241VPGSSLNSLN NLNNLSSPSL NSAVPTPACP YAPPTPPYVY RDTCNSSLAS LRLKAKQHSS301FGYASVQNPA SNLSACQYAV DRPVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
324 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.