Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PITPNM3 / NIR1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109287-100
Catalog Number: LS-G109287
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NIR1(PITPNM3) (NM_031220), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CORD5 | NIR-1 | NIR1 | PITPnm 3 | PITPNM3 | Retinal degeneration B alpha 3 | PITPNM family member 3 | RDGBA3
- Origin Species
- Human
- NCBI GenBank
- NM_031220
- SwissProt
- Q9BZ71
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 106.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKAGRAGGP PPGGGAPWHL RNVLSDSVES SDDEFFDARE EMAEGKNAIL IGMSQWNSND61LVEQIETMGK LDEHQGEGTA PCTSSILQEK QRELYRVSLR RQRFPAQGSI EIHEDSEEGC121PQRSCKTHVL LLVLHGGNIL DTGAGDPSCK AADIHTFSSV LEKVTRAHFP AALGHILIKF181VPCPAICSEA FSLVSHLNPY SHDEGCLSSS QDHVPLAALP LLAISSPQYQ DAVATVIERA241NQVYREFLKS SDGIGFSGQV CLIGDCVGGL LAFDAICYSA GPSGDSPASS SRKGSISSTQ301DTPVAVEEDC SLASSKRLSK SNIDISSGLE DEEPKRPLPR KQSDSSTYDC EAITQHHAFL361SSIHSSVLKD ESETPAAGGP QLPEVSLGRF DFDVSDFFLF GSPLGLVLAM RRTVLPGLDG421FQVRPACSQV YSFFHCADPS ASRLEPLLEP KFHLVPPVSV PRYQRFPLGD GQSLLLADAL481HTHSPLFLEG SSRDSPPLLD APASPPQASR FQRPGRRMSE GSSHSESSES SDSMAPVGAS541RITAKWWGSK RIDYALYCPD VLTAFPTVAL PHLFHASYWE STDVVAFILR QVMRYESVNI601KESARLDPAA LSPANPREKW LRKRTQVKLR NVTANHRAND VIAAEDGPQV LVGRFMYGPL661DMVALTGEKV DILVMAEPSS GRWVHLDTEI TNSSGRITYN VPRPRRLGVG VYPVKMVVRG721DQTCAMSYLT VLPRGMECVV FSIDGSFAAS VSIMGSDPKV RPGAVDVVRH WQDLGYMILY781ITGRPDMQKQ RVVSWLSQHN FPQGMIFFSD GLVHDPLRQK AIFLRNLMQE CFIKISAAYG841STKDISVYSV LGLPASQIFI VGRPTKKYQT QCQFLSEGYA AHLAALEASH RSRPKKNNSR901MILRKGSFGL HAQPEFLRKR NHLRRTMSVQ QPDPPAANPK PERAQSQPES DKDHERPLPA961LSWARGPPKF ESVPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
974 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.