Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PITPNM2 / NIR3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110951-100
Catalog Number: LS-G110951
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PITPNM2 (NM_020845), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NIR3 | PITPnm 2 | PITPNM2 | RDGBA2 | Retinal degeneration B alpha 2 | RDGB2 | KIAA1457 | NIR-3
- Origin Species
- Human
- NCBI GenBank
- AB040890
- SwissProt
- Q9BZ72
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 148.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIIKEYRIPL PMTVEEYRIA QLYMIQKKSR NETYGEGSGV EILENRPYTD GPGGSGQYTH61KVYHVGMHIP SWFRSILPKA ALRVVEESWN AYPYTRTRFT CPFVEKFSID IETFYKTDAG121ENPDVFNLSP VEKNQLTIDF IDIVKDPVPH NEYKTEEDPK LFQSTKTQRG PLSENWIEEY181KKQVFPIMCA YKLCKVEFRY WGMQSKIERF IHDTGLRRVM VRAHRQAWCW QDEWYGLSME241NIRELEKEAQ LMLSRKMAQF NEDGEEATEL VKHEAVSDQT SGEPPEPSSS NGEPLVGRGL301KKQWSTSSKS SRSSKRGASP SRHSISEWRM QSIARDSDES SDDEFFDAHE DLSDTEEMFP361KDITKWSSND LMDKIESPEP EDTQDGLYRQ GAPEFRVASS VEQLNIIEDE VSQPLAAPPS421KIHVLLLVLH GGTILDTGAG DPSSKKGDAN TIANVFDTVM RVHYPSALGR LAIRLVPCPP481VCSDAFALVS NLSPYSHDEG CLSSSQDHIP LAALPLLATS SPQYQEAVAT VIQRANLAYG541DFIKSQEGMT FNGQVCLIGD CVGGILAFDA LCYSNQPVSE SQSSSRRGSV VSMQDNDLLS601PGILMNAAHC CGGGGGGGGG GGSSGGGGSS GGSSLESSRH LSRSNVDIPR SNGTEDPKRQ661LPRKRSDSST YELDTIQQHQ AFLSSLHASV LRTEPCSRHS SSSTMLDGTG ALGRFDFEIT721DLFLFGCPLG LVLALRKTVI PALDVFQLRP ACQQVYNLFH PADPSASRLE PLLERRFHAL781PPFSVPRYQR YPLGDGCSTL LADVLQTHNA AFQEHGAPSS PGTAPASRGF RRASEISIAS841QVSGMAESYT ASSIAQKAPD ALSHTPSVRR LSLLALPAPS PTTPGPHPPA RKASPGLERA901PGLPELDIGE VAAKWWGQKR IDYALYCPDA LTAFPTVALP HLFHASYWES TDVVSFLLRQ961VMRHDNSSIL ELDGKEVSVF TPSKPREKWQ RKRTHVKLRN VTANHRINDA LANEDGPQVL1021TGRFMYGPLD MVTLTGEKVD VHIMTQPPSG EWLYLDTLVT NNSGRVSYTI PESHRLGVGV1081YPIKMVVRGD HTFADSYITV LPKGTEFVVF SIDGSFAASV SIMGSDPKVR AGAVDVVRHW1141QDLGYLIIYV TGRPDMQKQR VVAWLAQHNF PHGVVSFCDG LVHDPLRHKA NFLKLLISEL1201HLRVHAAYGS TKDVAVYSAI SLSPMQIYIV GRPTKKLQQQ CQFITDGYAA HLAQLKYSHR1261ARPARNTATR MALRKGSFGL PGQGDFLRSR NHLLRTISAQ PSGPSHRHER TQSQADGEQR1321GQRSMSVAAG CWGRAMTGRL EPGAAAGPKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1349 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.