Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PIPOX / Sarcosine Oxidase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112625-100
Catalog Number: LS-G112625
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sarcosine Oxidase(PIPOX) (NM_016518), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- L-pipecolic acid oxidase | LPIPOX | Peroxisomal sarcosine oxidase | PIPOX | Pipecolic acid oxidase | Sarcosine Oxidase | PSO | L-pipecolate oxidase
- Origin Species
- Human
- NCBI GenBank
- NM_016518
- SwissProt
- Q9P0Z9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAQKDLWDA IVIGAGIQGC FTAYHLAKHR KRILLLEQFF LPHSRGSSHG QSRIIRKAYL61EDFYTRMMHE CYQIWAQLEH EAGTQLHRQT GLLLLGMKEN QELKTIQANL SRQRVEHQCL121SSEELKQRFP NIRLPRGEVG LLDNSGGVIY AYKALRALQD AIRQLGGIVR DGEKVVEINP181GLLVTVKTTS RSYQAKSLVI TAGPWTNQLL RPLGIEMPLQ TLRINVCYWR EMVPGSYGVS241QAFPCFLWLG LCPHHIYGLP TGEYPGLMKV SYHHGNHADP EERDCPTART DIGDVQILSS301FVRDHLPDLK PEPAVIESCM YTNTPDEQFI LDRHPKYDNI VIGAGFSGHG FKLAPVVGKI361LYELSMKLTP SYDLAPFRIS RFPSLGKAHL TRTRPLEQKL ISEEDLAAND ILDYKDDDDK421V
421 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.