Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PIKFYVE / PIP5K Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106117-100
Catalog Number: LS-G106117
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIP5K3(PIKFYVE) (NM_152671), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAB1 | KIAA0981 | p235 | PIKFYVE | ZFYVE29 | PIP5K3 | PIP5K | PIPkin-III | Type III PIP kinase
- Origin Species
- Human
- NCBI GenBank
- NM_015040
- SwissProt
- Q9Y2I7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATDDKTSPT LDSANDLPRS PTSPSHLTHF KPLTPDQDEP PFKSAYSSFV NLFRFNKERA61EGGQGEQQPL SGSWTSPQLP SRTQSVRSPT PYKKQLNEEL QRRSSALGDL RACTYCRKIA121LSYAHSTDSN SIGEDLNALS DSACSVSVLD PSEPRTPVGS RKASRNIFLE DDLAWQSLIH181PDSSNTPLST RLVSVQEDAG KSPARNRSAS ITNLSLDRSG SPMVPSYETS VSPQANRTYV241RTETTEDERK ILLDSVQLKD LWKKICHHSS GMEFQDHRYW LRTHPNCIVG KELVNWLIRN301GHIATRAQAI AIGQAMVDGR WLDCVSHHDQ LFRDEYALYR PLQSTEFSET PSPDSDSVNS361VEGHSEPSWF KDIKFDDSDT EQIAEEGDDN LANSASPSKR TSVSSFQSTV DSDSAASISL421NVELDNVNFH IKKPSKYPHV PPHPADQKGR RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
451 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.