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PIK3R6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIK3R6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PIK3R6 antibodies →
$725.00each
Catalog Number: LS-G121694-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIK3R6 (NM_001010855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESSDVELDL QRSVQAVLRE LSTQAPALQS NQGMWRWSLH KKVERDPGKS PVLVRILLRE
61LEKAESQDLR HVIIPLLHTV MYVLTKATGI TEELYQRIYA FCTRLLTLPT PYCTVALDCA
121IRLKTEMAVP GTLYQRMVIA EQNLTNELYP YQERVFLFVD PELVSASVCS ALLLEIEAAQ
181AQQTPETCMR HVVSHALQAA LGEACHAGAL HRKLQASPRR TLEHYFHAVV AALEQMASEA
241SPSREGHVER LEEIYCSLLG PAAGRCGGDL VQERPPSIPL PSPYITFHLW TGEEQLWKEL
301VLFLRPRSQL RLSADLEVLD LQGLRPDREL ARVSVLSTDS GIERDLPTGA DELPAPGSPE
361MERAGLQRKG GIKKRAWPLD FLMPGSWDGP PGLHRRTGRP SGDGEMLPGV SRLHTARVLV
421LGDDRMLGRL AQAYHRLRKR ETQKFCLTPR LSLQLYYIPV LAPEKPAASR QPELGELATF
481LGRVDPWYQS NVNTLCPAIH KLAEMPPSLD TSRTVDPFIL DVITYYIRMG TQPIYFQIYT
541VKIFFSDLSQ DPTEDIFLIE LKVKIQDSKF PKDGFSPRRR GVAEGPGAEL SLCYQKALLS
601HRPREVTVSL RATGLILKAI PASDTEVSGS SHCPLPAAPV TDHTCLNVNV TEVVKSSNLA
661GKSFSTVTNT FRTNNIQIQS RDQRLLTLSL DKDDQRTFRD VVRFEVAPCP EPCSGAQKSK
721APWLNLHGQQ EVEAIKAKPK PLLMPINTFS GIVQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

754 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.