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PIK3R5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIK3R5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101194-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIK3R5 (NM_014308), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FOAP-2 | PIK3R5 | PtdIns-3-kinase p101 | p101-PI3K | PI3-kinase p101 subunit | Protein FOAP-2 | p101
Origin Species
Human
NCBI GenBank
NM_014308
SwissProt
Q8WYR1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MQPGATTCTE DRIQHALERC LHGLSLSRRS TSWSAGLCLN CWSLQELVSR DPGHFLILLE
61QILQKTREVQ EKGTYDLLTP LALLFYSTVL CTPHFPPDSD LLLKAASTYH RFLTWPVPYC
121SICQELLTFI DAELKAPGIS YQRLVRAEQG LPIRSHRSST VTVLLLNPVE VQAEFLAVAN
181KLSTPGHSPH SAYTTLLLHA FQATFGAHCD VPGLHCRLQA KTLAELEDIF TETAEAQELA
241SGIGDAAEAR RWLRTKLQAV GEKAGFPGVL DTAKPGKLHT IPIPVARCYT YSWSQDSFDI
301LQEILLKEQE LLQPGILGDD EEEEEEEEEV EEDLETDGHC AERDSLLSTS SLASHDSTLS
361LASSQASGPA LSRHLLTSFV SGLSDGMDSG YVEDSEESSS EWPWRRGSQE RRGHRRPGQK
421FIRIYKLFKS TSQLVLRRDS RSLEGSSDTA LPLRRAGSLC SPLDEPVSPP SRAQRSRSLP
481QPKLGTQLPS WLLAPASRPQ RRRPFLSGDE DPKASTLRVV VFGSDRISGK VARAYSNLRR
541LENNRPLLTR FFKLQFFYVP VKRSHGTSPG ACPPPRSQTP SPPTDSPRHA SPGELGTTPW
601EESTNDISHY LGMLDPWYER NVLGLMHLPP EVLCQQSLKA EAQALEGSPT QLPILADMLL
661YYCRFAARPV LLQVYQTELT FITGEKTTEI FIHSLELGHS AATRAIKASG PGSKRLGIDG
721DREAVPLTLQ IIYSKGAISG RSRWSNLEKV CTSVNLNKAC RKQEELDSSM EALTLNLTEV
781VKRQNSKSKK GFNQISTSQI KVDKVQIIGS NSCPFAVCLD QDERKILQSV VRCEVSPCYK
841PEKSDLSSPP QTPPDLPAQA APDLCSLLCL PIMTFSGALP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

880 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.