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PIGZ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIGZ Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109552-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIGZ (NM_025163), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GPI-MT-IV | HSMP3 | GPI mannosyltransferase 4 | GPI mannosyltransferase IV | PIGZ | PIG-Z | SMP3 | SMP3 homolog | SMP3 mannosyltransferase
Origin Species
Human
NCBI GenBank
NM_025163
SwissProt
Q86VD9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQICGSSVAS VAAGTSFQVL GPVCWQQLDL KMAVRVLWGG LSLLRVLWCL LPQTGYVHPD
61EFFQSPEVMA EDILGVQAAR PWEFYPSSSC RSVLFPLLIS GSTFWLLRLW EELGPWPGLV
121SGYALLVGPR LLLTALSFAL DGAVYHLAPP MGADRWNALA LLSGSYVTLV FYTRTFSNTI
181EGLLFTWLLV LVSSHVTWGP TRKEPAPGPR WRSWLLGGIV AAGFFNRPTF LAFAVVPLYL
241WGTRGATNPG LKSLTREALV LLPGATLTAA VFVATDSWYF SSPATSRNLV LTPVNFLHYN
301LNPQNLARHG THARLTHLAV NGFLLFGVLH AQALQAAWQQ LQVGLQASAQ MGLLRALGAR
361SLLSSPRSYL LLLYFMPLAL LSAFSHQEAR FLIPLLVPLV LLCSPQTQPV PWKGTVVLFN
421ALGALLFGCL HQGGLVPGLE YLEQVVHAPV LPSTPTHYTL LFTHTYMPPR HLLHLPGLGA
481PVEVVDIGGT EDWALCQTLK SFTRQPACQV AGGPWLCRLF VVTPGTTRRA VEKCSFPFKN
541ETLLFPHLTL EDPPALSSLL SGAWRDHLSL HIVELGEET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

579 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.