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PIGN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PIGN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PIGN antibodies →
$504.00each
Catalog Number: LS-G114436-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIGN (NM_012327), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MCAHS | MCAHS1 | MDC4 | MCD4 | MCD4 homolog | PIG-N | PIGN
Origin Species
Human
NCBI GenBank
NM_176787
SwissProt
O95427

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLFFTLGLL IHFVFFASIF DIYFTSPLVH GMTPQFTPLP PPARRLVLFV ADGLRADALY
61ELDENGNSRA PFIRNIIMHE GSWGISHTRV PTESRPGHVA LIAGFYEDVS AVAKGWKENP
121VEFDSLFNES KYTWSWGSPD ILPMFAKGAS GDHVYTYSYD AKREDFGAQD ATKLDTWVFD
181NVKDFFHHAR NNQSLFSKIN EEKIVFFLHL LGIDTNGHAH RPSSRDYKDN IKKVDDGVKE
241IVSMFNHFYG NDGKTTFIFT SDHGMTDWGS HGAGHPSETL TPLVTWGAGI KYPQRVSAQQ
301FDDAFLKEWR LENWKRLDVN QADIAPLMTS LIGVPFPLNS VGILPVDYLN NTDLFKAESM
361FTNAVQILEQ FKVKMTQKKE VTLPFLFTPF KLLSDSKQFN ILRKARSYIK HRKFDEVVSL
421CKELIHLALK GLSYYHTYDR FFLGVNVVIG FVGWISYASL LIIKSHSNLI KGVSKEVKKP
481SHLLPCSFVA IGILVAFFLL IQACPWTYYV YGLLPLPIWY AVLREFQVIQ DLVVSVLTYP
541LSHFVGYLLA FTLGIEVLVL SFFYRYMLTA GLTAFAAWPF LTRLWTRAKM TSLSWTFFSL
601LLAVFPLMPV VGRKPDIFLV MGAGLLVLLL SLCVVTSLMK RKDSFIKEEL LVHLLQVLST
661VLSMYVVYST QSSLLRKQGL PLMNQIISWA TLASSLVVPL LSSPVLFQRL FSILLSLMST
721YLLLSTGYEA LFPLVLSCLM FVWINIEQET LQQSGVCCKQ KLTSIQFSYN TDITQFRQLY
781LDDIRRAFFL VFFLVTAFFG TGNIASINSF DLASVYCFLT VFSPFMMGAL MMWKILIPFV
841LVMCAFEAVQ LTTQLSSKSL FLIVLVISDI MALHFFFLVK DYGSWLDIGT SISHYVIVMS
901MTIFLVFLNG LAQLLTTKKL RLCGKPKSHF M
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

931 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.