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PI4KA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PI4KA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G99821-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PI4KA (NM_002650), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PI4K-ALPHA | Pi4K230 | PI4KA | PIK4CA | PI4-kinase alpha | PIK4 | PtdIns-4-kinase alpha
NCBI GenBank
NM_002650
SwissProt
P42356

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MREMAGAWHM TVEQKFGLFS AEIKEADPLA ASEASQPKPC PPEVTPHYIW IDFLVQRFEI
61AKYCSSDQVE IFSSLLQRSM SLNIGGAKGS MNRHVAAIGP RFKLLTLGLS LLHADVVPNA
121TIRNVLREKI YSTAFDYFSC PPKFPTQGEK RLREDISIMI KFWTAMFSDK KYLTASQLVP
181PDNQDTRSNL DITVGSRQQA TQGWINTYPL SSGMSTISKK SGMSKKTNRG SQLHKYYMKR
241RTLLLSLLAT EIERLITWYN PLSAPELELD QAGENSVANW RSKYISLSEK QWKDNVNLAW
301SISPYLAVQL PARFKNTEAI GNEVTRLVRL DPGAVSDVPE AIKFLVTWHT IDADAPELSH
361VLCWAPTDPP TGLSYFSSMY PPHPLTAQYG VKVLRSFPPD AILFYIPQIV QALRYDKMGY
421VREYILWAAS KSQLLAHQFI WNMKTNIYLD EEGHQKDPDI GDLLDQLVEE ITGSLSGPAK
481DFYQREFDFF NKITNVSAII KPYPKGDERK KACLSALSEV KVQPGCYLPS NPEAIVLDID
541YKSGTPMQSA AKAPYLAKFK VKRCGVSELE KEGLRCRSDS EDECSTQEAD GQKISWQAAI
601FKVGDDCRQD MLALQIIDLF KNIFQLVGLD LFVFPYRVVA TAPGCGVIEC IPDCTSRDQL
661GRQTDFGMYD YFTRQYGDES TLAFQQARYN FIRSMAAYSL LLFLLQIKDR HNGNIMLDKK
721GHIIHIDFGF MFESSPGGNL GWEPDIKLTD EMVMIMGGKM EATPFKWFME MCVRGYLAVR
781PYMDAVVSLV TLMLDTGLPC FRGQTIKLLK HRFSPNMTER EAANFIMKVI QSCFLSNRSR
841TYDMIQYYQN DIPY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

854 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.