Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PI3 / Elafin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99820-100
Catalog Number: LS-G99820
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PI3(PI3) (NM_002638), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cementoin | Elafin | Elastase-specific inhibitor | ESI | PI-3 | PI3 | Protease inhibitor WAP3 | Trappin-2 | Peptidase inhibitor 3 | Protease inhibitor 3 | SKALP | WAP3 | WFDC14 | Pre-elafin
- NCBI GenBank
- NM_002638
- SwissProt
- P19957
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 12.27 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRASSFLIVV VFLIAGTLVL EAAVTGVPVK GQDTVKGRVP FNGQDPVKGQ VSVKGQDKVK61AQEPVKGPVS TKPGSCPIIL IRCAMLNPPN RCLKDTDCPG IKKCCEGSCG MACFVPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
117 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.