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PI16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PI16 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PI16 antibodies →
$504.00each
Catalog Number: LS-G105812-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Peptidase inhibitor 16(PI16) (NM_153370), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CRISP-9 | MSMBBP | PSP94-binding protein | PI-16 | PI16 | Protease inhibitor 16 | CRISP9 | DJ90K10.5 | Peptidase inhibitor 16 | PSPBP
Origin Species
Human
SwissProt
Q6UXB8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHGSCSFLML LLPLLLLLVA TTGPVGALTD EEKRLMVELH NLYRAQVSPP ASDMLHMRWD
61EELAAFAKAY ARQCVWGHNK ERGRRGENLF AITDEGMDVP LAMEEWHHER EHYNLSAATC
121SPGQMCGHYT QVVWAKTERI GCGSHFCEKL QGVEETNIEL LVCNYEPPGN VKGKRPYQEG
181TPCSQCPSGY HCKNSLCEPI GSPEDAQDLP YLVTEAPSFR ATEASDSRKM GTPSSLATGI
241PAFLVTEVSG SLATKALPAV ETQAPTSLAT KDPPSMATEA PPCVTTEVPS ILAAHSLPSL
301DEEPVTFPKS THVPIPKSAD KVTDKTKVPS RSPENSLDPK MSLTGARELL PHAQEEAEAE
361AELPPSSEVL ASVFPAQDKP GELQATLDHT GHTSSKSLPN FPNTSATANA TGGRALALQS
421SLPGAEGPDK PSVVSGLNSG PGHVWGPLLG LLLLPPLVLA GIF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

463 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.