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PHTF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHTF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111164-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHTF2 (NM_020432), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PHTF2
Origin Species
Human
SwissProt
Q8N3S3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPKVTDAIV WYQKKEFLSV ATTAPGPQQV LPGYCQCSLK DQGLFIQCLI GAYDQQIWEK
61SVEQREIKFI KLGLRNKPKK TAHVKPDLID VDLVRGSAFA KAKPESPWTS LTRKGIVRVV
121FFPFFFRWWL QVTSKVIFFW LLVLYLLQVA AIVLFCSTSS PHSIPLTEVI GPIWLMLLLG
181TVHCQIVSTR TPKPPLSTGG KRRRKLRKAA HLEVHREGDG SSTTDNTQEG AVQNHGTSTS
241HSVGTVFRDL WHAAFFLSGS KKAKNSIDKS TETDNGYVSL DGKKTVKSGE DGIQNHEPQC
301ETIRPEETAW NTGTLRNGPS KDTQRTITNV SDEVSSEEGP ETGYSLRRHV DRTSEGVLRN
361RKSHHYKKHY PNEDAPKSGT SCSSRCSSSR QDSESARPES ETEDVLWEDL LHCAECHSSC
421TSETDVENHQ INPCVKKEYR DDPFHQSHLP WLHSSHPGLE KISAIVWEGN DCKKADMSVL
481EISGMIMNRV NSHIPGIGYQ IFGNAVSLIL GLTPFVFRLS QATDLEQLTA HSASELYVIA
541FGSNEDVIVL SMVIISFVVR VSLVWIFFFL LCVAERTYKQ RLLFAKLFGH LTSARRARKS
601EVPHFRLKKV QNIKMWLSLR SYLKRRGPQR SVDVIVSSAF LLTISVVFIC CAQLLHVHEI
661FLDCHYNWEL VIWCISLTLF LLRFVTLGSE TSKKYSNTSI LLTEQINLYL KMEKKPNKKE
721ELTLVNNVLK LATKLLKELD SPFRLYGLTM NPLLYNITQV VILSAVSGVI SDLLGFNLKL
781WKIKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

785 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.