Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PHLPP2 / PHLPPL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113524-100
Catalog Number: LS-G113524
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHLPP2 (NM_015020), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PHLPPL | PHLPP-like | KIAA0931 | PHLPP2
- Origin Species
- Human
- NCBI GenBank
- NM_015020
- SwissProt
- Q6ZVD8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 146.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKRNGSRNCL NRRSRFGSRE RDWLREDVKR GCVYLYGADT TTATTTTTTS SSSSSSSSSS61DLHLVLCTVE TPASEICAGE GRESLYLQLH GDLVRRLEPT ERPLQIVYDY LSRLGFDDPV121RIQEEATNPD LGCMIRFYGE KPCHMDRLDR ILLSGIYNVR KGKTQLHKWA ERLVVLCGTC181LIVSSVKDCQ TGKMHILPLV GGKIEEVKRR QYSLAFSSAG AQAQTYHVSF ETLAEYQRWQ241RQASKVVSQR ISTVDLSCYS LEEVPEHLFY SQDITYLNLR HNFMQLERPG GLDTLYKFSQ301LKGLNLSHNK LGLFPILLCE ISTLTELNLS CNGFHDLPSQ IGNLLNLQTL CLDGNFLTTL361PEELGNLQQL SSLGISFNNF SQIPEVYEKL TMLDRVVMAG NCLEVLNLGV LNRMNHIKHV421DLRMNHLKTM VIENLEGNKH ITHVDLRDNR LTDLDLSSLC SLEQLHCGRN QLRELTLSGF481SLRTLYASSN RLTAVNVYPV PSLLTFLDLS RNLLECVPDW ACEAKKIEVL DVSYNLLTEV541PVRILSSLSL RKLMLGHNHV QNLPTLVEHI PLEVLDLQHN ALTRLPDTLF SKALNLRYLN601ASANSLESLP SACTGEESLS MLQLLYLTNN LLTDQCIPVL VGHLHLRILH LANNQLQTFP661ASKLNKLEQL EELNLSGNKL KTIPTTIANC KRLHTLVAHS NNISIFPEIL QLPQIQFVDL721SCNDLTEILI PEALPATLQD LDLTGNTNLV LEHKTLDIFS HITTLKIDQK PLPTTDSTVT781STFWSHGLAE MAGQRNKLCV SALAMDSFAE GVGAVYGMFD GDRNEELPRL LQCTMADVLL841EEVQQSTNDT VFMANTFLVS HRKLGMAGQK LGSSALLCYI RPDTADPASS FSLTVANVGT901CQAVLCRGGK PVPLSKVFSL EQDPEEAQRV KDQKAIITED NKVNGVTCCT RMLGCTYLYP961WILPKPHISS TPLTIQDELL ILGNKALWEH LSYTEAVNAV RHVQDPLAAA KKLCTLAQSY1021GCQDNVGAMV VYLNIGEEGC TCEMNGLTLP GPVGFASTTT IKDAPKPATP SSSSGIASEF1081SSEMSTSEVS SEVGSTASDE HNAGGLDTAL LPRPERRCSL HPTPTSGLFQ RQPSSATFSS1141NQSDNGLDSD DDQPVEGVIT NGSKVEVEVD IHCCRGRDLE NSPPLIESSP TLCSEEHARG1201SCFGIRRQNS VNSGMLLPMS KDRMELQKSP STSCLYGKKL SNGSIVPLED SLNLIEVATE1261VPKRKTGYFA APTQMEPEDQ FVVPHDLEEE VKEQMKQHQD SRLEPEPHEE DRTEPPEEFD1321TALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1323 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.