LSBio
PHLDB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHLDB3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHLDB3 antibodies →
$725.00each
Catalog Number: LS-G103525-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHLDB3 (NM_198850), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PHLDB3
Origin Species
Human
NCBI GenBank
NM_198850
SwissProt
Q6NSJ2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTRSSPEEG TPPPLVPECD VEVQPQGHPE ESREQEASEV LAEPSSRGGA EQQAEEEEVG
61EGSSTESSRD APEATPPIAM AATPPASTSS REGVRGAARR LQGQQLEALT RVALMEQRVK
121ELQRQRKELR IEMEVEVALL RGELAGERVA ARREEEQLRE LLEQQAASEQ RGRQQREQEQ
181RRLSQERDRL EGLRQRLRKA QGQLDSQPED QRERLLQGVQ EMREQLDVAQ RAYEDLEFQQ
241LERESRQEEE DRDSPGPQVP DPKVQELQAS MAQHRRGALQ HRIRVLEEQL KSLGEQMAAE
301SRGLSRKKEE ALQALSQERS RLLELNCLQG TPGGDFSEPN PALTKLLFTQ KTDRQLLVLQ
361DAVAHSAATP TSSCLFSVHS SLQGSIGLQR TGSLPRKRGE RGSQRGSPRP LSFHCTESLE
421ASALPPAVGD SGRYPLYQLL NCGRGNSCGA IHPDIAHMER LLQQAMAERE RLLKAREGTR
481RGTEGSSGPA VPAITAPPTP PHPPGPRILD LRQHLEGWGH NPENCPHVQV SGCCCRGPLV
541KMGGRIKTWR KRWFCFDRQA RRLAYYADKE ETKLKGVIYF QAIEEVYYDH LRCAFKSPNP
601RLTFCVKTYE RLFYMVAPSP EAMRIWMDVI VTAADENHAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

640 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.