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PHLDB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHLDB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHLDB2 antibodies →
$725.00each
Catalog Number: LS-G125282-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHLDB2 (NM_001134437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001134437

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
139.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEEDTKREV PKEDGVGDVQ HFDSSKIMEE HSYIQKELDL QNGSLEEDSV VHSVENDSQN
61MMESLSPKKY SSSLRFKANG DYSGSYLTLS QPVPAKRSPS PLGTSVRSSP SLAKIQGSKQ
121FSYDGTDKNI PMKPPTPLLN TTSSLSGYPL GRADFDHYTG RDSERALRLS EKPPYSKYSS
181RHKSHDNVYS LGGLEGRKAS GSLLAMWNGS SLSDAGPPPI SRSGAASMPS SPKQARKMSI
241QDSLALQPKL TRHKELASEN INLRTRKYSS SSLSHMGAYS RSLPRLYRAT ENQLTPLSLP
301PRNSLGNSKR TKLGEKDLPH SVIDNDNYLN FSSLSSGALP YKTSASEGNP YVSSTLSVPA
361SPRVARKMLL ASTSSCASDD FDQASYVGTN PSHSLLAGES DRVFATRRNF SCGSVEFDEA
421DLESLRQASG TPQPALRERK SSISSISGRD DLMDYHRRQR EERLREQEME RLERQRLETI
481LSLCAEYTKP DSRLSTGTTV EDVQKINKEL EKLQLSDEES VFEEALMSPD TRYRCHRKDS
541LPDADLASCG SLSQSSASFF TPRSTRNDEL LSDLTRTPPP PSSTFPKASS ESSYLSILPK
601TPEGISEEQR SQELAAMEET RIVILNNLEE LKQKIKDIND QMDESFRELD MECALLDGEQ
661KSETTELMKE KEILDHLNRK IAELEKNIVG EKTKDADLLD VESKHFEDLE FQQLEHESRL
721DEEKENLTQQ LLREVAEYQR NIVSRKEKIS ALKKQANHIV QQAQREQDHF VKEKNNLIMM
781LQREKENLCN LEKKYSSLSG GKGFPVNPNT LKEGYISVNE INEPCGNSTN LSPSTQFPAD
841ADAVATEPAT AVLASQPQSK EHFRSLEERK KQHKEGLYLS DTLPRKKTTS SISPHFSSAT
901MGRSITPKAH LPLGQSNSCG SVLPPSLAAM AKDSESRRML RGYNHQQMSE GHRQKSEFYN
961RTASESNVYL NSFHYPDHSY KDQAFDTLSL DSSDSMETSI SACSPDNISS ASTSNIARIE
1021EMERLLKQAH AEKTRLLESR EREMEAKKRA LEEEKRRREI LEKRLQEETS QRQKLIEKEV
1081KIRERQRAQA RPLTRYLPVR KEDFDLRSHV ETAGHNIDTC YHVSITEKTC RGFLIKMGGK
1141IKTWKKRWFV FDRNKRTFSY YADKHETKLK GVIYFQAIEE VYYDHLKNAN KSPNPLLTFS
1201VKTHDRIYYM VAPSPEAMRI WMDVIVTGAE GYTHFLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1237 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.