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PHLDB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHLDB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHLDB1 antibodies →
$725.00each
Catalog Number: LS-G113418-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHLDB1 (NM_015157), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0638 | LL5A | LL5alpha | PHLDB1 | Protein LL5-alpha
Origin Species
Human
SwissProt
Q86UU1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
151 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDALNRNQIG PGCQTQTMVQ KGPLDLIETG KGLKVQTDKP HLVSLGSGRL STAITLLPLE
61EGRTVIGSAA RDISLQGPGL APEHCYIENL RGTLTLYPCG NACTIDGLPV RQPTRLTQGC
121MLCLGQSTFL RFNHPAEAKW MKSMIPAGGR APGPPYSPVP AESESLVNGN HTPQTATRGP
181SACASHSSLV SSIEKDLQEI MDSLVLEEPG AAGKKPAATS PLSPMANGGR YLLSPPTSPG
241AMSVGSSYEN TSPAFSPLSS PASSGSCASH SPSGQEPGPS VPPLVPARSS SYHLALQPPQ
301SRPSGARSES PRLSRKGGHE RPPSPGLRGL LTDSPAATVL AEARRATESP RLGGQLPVVA
361ISLSEYPASG ALSQPTSIPG SPKFQPPVPA PRNKIGTLQD RPPSPFREPP GSERVLTTSP
421SRQLVGRTFS DGLATRTLQP PESPRLGRRG LDSMRELPPL SPSLSRRALS PLPTRTTPDP
481KLNREVAESP RPRRWAAHGA SPEDFSLTLG ARGRRTRSPS PTLGESLAPH KGSFSGRLSP
541AYSLGSLTGA SPCQSPCVQR KLSSGDLRVP VTRERKNSIT EISDNEDDLL EYHRRQRQER
601LREQEMERLE RQRLETILNL CAEYSRADGG PEAGELPSIG EATAALALAG RRPSRGLAGA
661SGRSSEEPGV ATQRLWESME RSDEENLKEE CSSTESTQQE HEDAPSTKLQ GEVLALEEER
721AQVLGHVEQL KVRVKELEQQ LQESAREAEM ERALLQGERE AERALLQKEQ KAVDQLQEKL
781VALETGIQKE RDKEAEALET ETKLFEDLEF QQLERESRVE EERELAGQGL LRSKAELLRS
841IAKRKERLAI LDSQAGQIRA QAVQESERLA RDKNASLQLL QKEKEKLTVL ERRYHSLTGG
901RPFPKTTSTL KEMEKLLLPA VDLEQWYQEL MAGLGTGPAA ASPHSSPPPL PAKASRQLQV
961YRSKMDGEAT SPLPRTRSGP LPSSSGSSSS SSQLSVATLG RSPSPKSALL TQNGTGSLPR
1021NLAATLQDIE TKRQLALQQK GQQVIEEQRR RLAELKQKAA AEAQCQWDAL HGAAPFPAGP
1081SGFPPLMHHS ILHHLPAGRE RGEEGEHAYD TLSLESSDSM ETSISTGGNS ACSPDNMSSA
1141SGLDMGKIEE MEKMLKEAHA EKNRLMESRE REMELRRQAL EEERRRREQV ERRLQSESAR
1201RQQLVEKEVK MREKQFSQAR PLTRYLPIRK EDFDLKTHIE SSGHGVDTCL HVVLSSKVCR
1261GYLVKMGGKI KSWKKRWFVF DRLKRTLSYY VDKHETKLKG VIYFQAIEEV YYDHLRSAAK
1321KRFFRFTMVT ESPNPALTFC VKTHDRLYYM VAPSAEAMRI WMDVIVTGAE GYTQFMN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1377 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.