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PHKB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHKB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHKB antibodies →
$504.00each
Catalog Number: LS-G120749-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Phosphorylase B(PHKB) (NM_001031835), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Phosphorylase kinase | beta | PHKB
Origin Species
Human
NCBI GenBank
NM_000293
SwissProt
Q93100

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACSPDAVVS PSSAFLRSGS VYEPLKSINL PRPDNETLWD KLDHYYRIVK STLLLYQSPT
61TGLFPTKTCG GDQKAKIQDS LYCAAGAWAL ALAYRRIDDD KGRTHELEHS AIKCMRGILY
121CYMRQADKVQ QFKQDPRPTT CLHSVFNVHT GDELLSYEEY GHLQINAVSL YLLYLVEMIS
181SGLQIIYNTD EVSFIQNLVF CVERVYRVPD FGVWERGSKY NNGSTELHSS SVGLAKAALE
241AINGFNLFGN QGCSWSVIFV DLDAHNRNRQ TLCSLLPRES RSHNTDAALL PCISYPAFAL
301DDEVLFSQTL DKVVRKLKGK YGFKRFLRDG YRTSLEDPNR CYYKPAEIKL FDGIECEFPI
361FFLYMMIDGV FRGNPKQVQE YQDLLTPVLH HTTEGYPVVP KYYYVPADFV EYEKNNPGSQ
421KRFPSNCGRD GKLFLWGQAL YIIAKLLADE LISPKDIDPV QRYVPLKDQR NVSMRFSNQG
481PLENDLVVHV ALIAESQRLQ VFLNTYGIQT QTPQQVEPIQ IWPQQELVKA YLQLGINEKL
541GLSGRPDRPI GCLGTSKIYR ILGKTVVCYP IIFDLSDFYM SQDVFLLIDD IKNALQFIKQ
601YWKMHGRPLF LVLIREDNIR GSRFNPILDM LAALKKGIIG GVKVHVDRLQ TLISGAVVEQ
661LDFLRISDTE ELPEFKSFEE LEPPKHSKVK RQSSTPSAPE LGQQPDVNIS EWKDKPTHEI
721LQKLNDCSCL ASQAILLGIL LKREGPNFIT KEGTVSDHIE RVYRRAGSQK LWSVVRRAAS
781LLSKVVDSLA PSITNVLVQG KQVTLGAFGH EEEVISNPLS PRVIQNIIYY KCNTHDEREA
841VIQQELVIHI GWIISNNPEL FSGMLKIRIG WIIHAMEYEL QIRGGDKPAL DLYQLSPSEV
901KQLLLDILQP QQNGRCWLNR RQIDGSLNRT PTGFYDRVWQ ILERTPNGII VAGKHLPQQP
961TLSDMTMYEM NFSLLVEDTL GNIDQPQYRQ IVVELLMVVS IVLERNPELE FQDKVDLDRL
1021VKEAFNEFQK DQSRLKEIEK QDDMTSFYNT PPLGKRGTCS YLTKAVMNLL LEGEVKPNND
1081DPCLIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1086 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.