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PHF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHF3 antibodies →
$725.00each
Catalog Number: LS-G113416-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF3 (NM_015153), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0244 | PHD finger protein 3 | PHF3
Origin Species
Human
NCBI GenBank
NM_015153
SwissProt
Q92576

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
229.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDIVDTFNHL IPTEHLDDAL FLGSNLENEV CEDFSASQNV LEDSLKNMLS DKDPMLGSAS
61NQFCLPVLDS NDPNFQMPCS TVVGLDDIMD EGVVKESGND TIDEEELILP NRNLRDKVEE
121NSVRSPRKSP RLMAQEQVRS LRQSTIAKRS NAAPLSNTKK ASGKTVSTAK AGVKQPERSQ
181VKEEVCMSLK PEYHKENRRC SRNSGQIEVV PEVSVSSSHS SVSSCLEMKD EDGLDSKHKC
241NNPGEIDVPS HELNCSLLSE TCVTIGEKKN EALMECKAKP VGSPLFKFSD KEEHEQNDSI
301SGKTGETVVE EMIATRKVEQ DSKETVKLSH EDDHILEDAG SSDISSDAAC TNPNKTENSL
361VGLPSCVDEV TECNLELKDT MGIADKTENT LERNKIEPLG YCEDAESNRQ LESTEFNKSN
421LEVVDTSTFG PESNILENAI CDVPDQNSKQ LNAIESTKIE SHETANLQDD RNSQSSSVSY
481LESKSVKSKH TKPVIHSKQN MTTDAPKKIV AAKYEVIHSK TKVNVKSVKR NTDVPESQQN
541FHRPVKVRKK QIDKEPKIQS CNSGVKSVKN QAHSVLKKTL QDQTLVQIFK PLTHSLSDKS
601HAHPGCLKEP HHPAQTGHVS HSSQKQCHKP QQQAPAMKTN SHVKEELEHP GVEHFKEEDK
661LKLKKPEKNL QPRQRRSSKS FSLDEPPLFI PDNIATIRRE GSDHSSSFES KYMWTPSKQC
721GFCKKPHGNR FMVGCGRCDD WFHGDCVGLS LSQAQQMGEE DKEYVCVKCC AEEDKKTEIL
781DPDTLENQAT VEFHSGDKTM ECEKLGLSKH TTNDRTKYID DTVKHKVKIL KRESGEGRNS
841SDCRDNEIKK WQLAPLRKMG QPVLPRRSSE EKSEKIPKES TTVTCTGEKA SKPGTHEKQE
901MKKKKVEKGV LNVHPAASAS KPSADQIRQS VRHSLKDILM KRLTDSNLKV PEEKAAKVAT
961KIEKELFSFF RDTDAKYKNK YRSLMFNLKD PKNNILFKKV LKGEVTPDHL IRMSPEELAS
1021KELAAWRRRE NRHTIEMIEK EQREVERRPI TKITHKGEIE IESDAPMKEQ EAAMEIQEPA
1081ANKSLEKPEG SEKQKEEVDS MSKDTTSQHR QHLFDLNCKI CIGRMAPPVD DLSPKKVKVV
1141VGVARKHSDN EAESIADALS STSNILASEF FEEEKQESPK STFSPAPRPE MPGTVEVEST
1201FLARLNFIWK GFINMPSVAK FVTKAYPVSG SPEYLTEDLP DSIQVGGRIS PQTVWDYVEK
1261IKASGTKEIC VVRFTPVTEE DQISYTLLFA YFSSRKRYGV AANNMKQVKD MYLIPLGATD
1321KIPHPLVPFD GPGLELHRPN LLLGLIIRQK LKRQHSACAS TSHIAETPES APPIALPPDK
1381KSKIEVSTEE APEEENDFFN SFTTVLHKQR NKPQQNLQED LPTAVEPLME VTKQEPPKPL
1441RFLPGVLIGW ENQPTTLELA NKPLPVDDIL QSLLGTTGQV YDQAQSVMEQ NTVKEIPFLN
1501EQTNSKIEKT DNVEVTDGEN KEIKVKVDNI SESTDKSAEI ETSVVGSSSI SAGSLTSLSL
1561RGKPPDVSTE AFLTNLSIQS KQEETVESKE KTLKRQLQED QENNLQDNQT SNSSPCRSNV
1621GKGNIDGNVS CSENLVANTA RSPQFINLKR DPRQAAGRSQ PVTTSESKDG DSCRNGEKHM
1681LPGLSHNKEH LTEQINVEEK LCSAEKNSCV QQSDNLKVAQ NSPSVENIQT SQAEQAKPLQ
1741EDILMQNIET VHPFRRGSAV ATSHFEVGNT CPSEFPSKSI TFTSRSTSPR TSTNFSPMRP
1801QQPNLQHLKS SPPGFPFPGP PNFPPQSMFG FPPHLPPPLL PPPGFGFAQN PMVPWPPVVH
1861LPGQPQRMMG PLSQASRYIG PQNFYQVKDI RRPERRHSDP WGRQDQQQLD RPFNRGKGDR
1921QRFYSDSHHL KRERHEKEWE QESERHRRRD RSQDKDRDRK SREEGHKDKE RARLSHGDRG
1981TDGKASRDSR NVDKKPDKPK SEDYEKDKER EKSKHREGEK DRDRYHKDRD HTDRTKSKR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2039 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.