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PHF23 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHF23 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHF23 antibodies →
$504.00each
Catalog Number: LS-G110009-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF23 (NM_024297), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PDH-containing protein JUNE-1 | PHD finger protein 23 | PHD finger protein 23a | PHF23 | HJUNE-1b
Origin Species
Human
NCBI GenBank
NM_024297
SwissProt
Q9BUL5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MLEAMAEPSP EDPPPTLKPE TQPPEKRRRT IEDFNKFCSF VLAYAGYIPP SKEESDWPAS
61GSSSPLRGES AADSDGWDSA PSDLRTIQTF VKKAKSSKRR AAQAGPTQPG PPRSTFSRLQ
121APDSATLLEK MKLKDSLFDL DGPKVASPLS PTSLTHTSRP PAALTPVPLS QGDLSHPPRK
181KDRKNRKLGP GAGAGFGVLR RPRPTPGDGE KRSRIKKSKK RKLKKAERGD RLPPPGPPQA
241PPSDTDSEEE EEEEEEEEEE EMATVVGGEA PVPVLPTPPE APRPPATVHP EGVPPADSES
301KEVGSTETSQ DGDASSSEGE MRVMDEDIMV ESGDDSWDLI TCYCRKPFAG RPMIECSLCG
361TWIHLSCAKI KKTNVPDFFY CQKCKELRPE ARRLGGPPKS GEP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

403 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.