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PHF21B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHF21B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHF21B antibodies →
$504.00each
Catalog Number: LS-G107383-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF21B (NM_138415), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BHC80L | KIAA1661 | PHD finger protein 4 | PHD finger protein 21B | PHF21B | PHF4
Origin Species
Human
NCBI GenBank
NM_138415
SwissProt
Q96EK2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELQSRPEAL AVELARHQNG DLKKQLHERQ PRIAALSDKQ ALGTITAVPV TGPQVSSLQR
61LAGQGAAVLP QVRPKTLIPD SLPVAPGRDR PPKQPPTFQK ATVVSVKNPS PALPTANNTV
121SHVPAPGSQP QALAEPAALA SPLSSAGVAY AIISTSPSNA AAMAPSTAVS VVSDSIKVQP
181LLISADNKPP PRLLSSPHPA THHCPLHPSS LPLTPPSPSL SPSPLHGIFQ VIIIQPQVQT
241QPESTAESRP PTEEPSQGAQ ATKKKKEDRP PTQENPEKIA FMVALGLVTT EHLEEIQSKR
301QERKRRSTAN PAYSGLLETE RKRLASNYLN NPLFLTARAN EDPCWKNEIT HDEHCAACKR
361GANLQPCGTC PGAYHLSCLE PPLKTAPKGV WVCPRCQQKA LKKDEGVPWT GMLAIVHSYV
421THKTVKEEEK QKLLQRGSEL QNEHQQLEER DRRLASAVQK CLELKTSLLA RQRGTQSSLD
481RLRALLRLIQ GEQLLQVTMT TTSPAPLLAG PWTKPSVAAT HPTVQHPQGH N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.