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PHF20L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHF20L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHF20L1 antibodies →
$725.00each
Catalog Number: LS-G108965-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF20L1 (NM_032205), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CGI-72 | PHD finger protein 20-like 1 | PHF20L1
Origin Species
Human
NCBI GenBank
NM_016018
SwissProt
A8MW92

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKKPPNRPG ITFEIGARLE ALDYLQKWYP SRIEKIDYEE GKMLVHFERW SHRYDEWIYW
61DSNRLRPLER PALRKEGLKD EEDFFDFKAG EEVLARWTDC RYYPAKIEAI NKEGTFTVQF
121YDGVIRCLKR MHIKAMPEDA KGQVKSQHPL SWCCPIDPAG SCNQSMGSED WIALVKAAAA
181AAAKNKTGSK PRTSANSNKD KDKDERKWFK VPSKKEETST CIATPDVEKK EDLPTSSETF
241GLHVENVPKM VFPQPESTLS NKRKNNQGNS FQAKRARLNK ITGLLASKAV GVDGAEKKED
301YNETAPMLEQ AISPKPQSQK KNEADISSSA NTQKPALLSS TLSSGKARSK KCKHESGDSS
361GCIKPPKSPL SPELIQVEDL TLVSQLSSSV INKTSPPQPV NPPRPFKHSE RRRRSQRLAT
421LPMPDDSVEK VSSPSPATDG KVFSISSQNQ QESSVPEVPD VAHLPLEKLG PCLPLDLSRG
481SEVTAPVASD SSYRNECPRA EKEDTQMLPN PSSKAIADGR GAPAAAGISK TEKKVKLEDK
541SSTAFGIRSW DFSALLMKIP SYSPISLSGL PES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

573 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.