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PHF12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHF12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHF12 antibodies →
$725.00each
Catalog Number: LS-G120940-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHF12 (NM_001033561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1523 | PHD finger protein 12 | PF1 | PHD factor 1 | PHF12
Origin Species
Human
NCBI GenBank
AI092429
SwissProt
Q96QT6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWEKMETKTI VYDLDTSGGL MEQIQALLAP PKTDEAEKRS RKPEKEPRRS GRATNHDSCD
61SCKEGGDLLC CDHCPAAFHL QCCNPPLSEE MLPPGEWMCH RCTVRRKKRE QKKELGHVNG
121LVDKSGKRTT SPSSDTDLLD RSASKTELKA IAHARILERR ASRPGTPTSS ASTETPTSEQ
181NDVDEDIIDV DEEPVAAEPD YVQPQLRRPF ELLIAAAMER NPTQFQLPNE LTCTTALPGS
241SKRRRKEETT GKNVKKTQHE LDHNGLVPLP VKVCFTCNRS CRVAPLIQCD YCPLLFHMDC
301LEPPLTAMPL GRWMCPNHIE HVVLNQKNMT LSNRCQVFDR FQDTVSQHVV KVDFLNRIHK
361KHPPNRRVLQ SVKRRSLKVP DAIKSQYQFP PPLIAPAAIR DGELICNGIP EESQMHLLNS
421EHLATQAEQQ EWLCSVVALQ CSILKHLSAK QMPSHWDSEQ TEKADIKPVI VTDSSVTTSL
481QTADKTPTPS HYPLSCPSGI STQNSLSCSP PHQSPALEDI GCSSCAEKSK KTPCGTANGP
541VNTEVKANGP HLYSSPTDST DPRRLPGANT PLPGLSHRQG WPRPLTPPAA GGLQNHTVGI
601IVKTENATGP SSCPQRSLVP VPSLPPSIPS SCASIENTST LQRKTVQSQI GPPLTDSRPL
661GSPPNATRVL TPPQAAGDGI LATTANQRFS SPAPSSDGKV SPGTLSIGSA LTVPSFPANS
721TAMVDLTNSL RAFMDVNGEI EINMLDEKLI KFLALQRIHQ LFPSRVQPSP GSVGTHQLAS
781GGHHIEVQRK EVQARAVFYP LLGLGGAVNM CYRTLYIGTG ADMDVCLTNY GHCNYVSGKH
841ACIFYDENTK HYELLNYSEH GTTVDNVLYS CDFSEKTPPT PPSSIVAKVQ SVIRRRRHQK
901QDEEPSEEAA MMSSQAQGPQ RRPCNCKASS SSLIGGSGAG WEGTALLHHG SYIKLGCLQF
961VFSITEFATK QPKGDASLLQ DGVLAEKLSL KPHQGPVLRS NSVP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1004 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.