Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PHC1 / EDR1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100294-100
Catalog Number: LS-G100294
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHC1(PHC1) (NM_004426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HPH1 | PH1 | PHC1 | Polyhomeotic-like 1 | Polyhomeotic-like protein 1 | RAE28 | EDR1
- Origin Species
- Human
- NCBI GenBank
- NM_004426
- SwissProt
- P78364
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METESEQNSN STNGSSSSGG SSRPQIAQMS LYERQAVQAL QALQRQPNAA QYFHQFMLQQ61QLSNAQLHSL AAVQQATIAA SRQASSPNTS TTQQQTTTTQ ASINLATTSA AQLISRSQSV121SSPSATTLTQ SVLLGNTTSP PLNQSQAQMY LRPQLGNLLQ VNRTLGRNVP LASQLILMPN181GAVAAVQQEV PSAQSPGVHA DADQVQNLAV RNQQASAQGP QMQGSTQKAI PPGASPVSSL241SQASSQALAV AQASSGATNQ SLNLSQAGGG SGNSIPGSMG PGGGGQAHGG LGQLPSSGMG301GGSCPRKGTG VVQPLPAAQT VTVSQGSQTE AESAAAKKAE ADGSGQQNVG MNLTRTATPA361PSQTLISSAT YTQIQPHSLI QQQQQIHLQQ KQVVIQQQIA IHHQQQFQHR QSQLLHTATH421LQLAQQQQQQ QQQQQQQQQP QATTLTAPQP PQVPPTQQVP PSQSQQQAQT LVVQPMLQSS481PLSLPPDAAP KPPIPIQSKP PVAPIKPPQL GAAKMSAAQQ PPPHIPVQVV GTRQPGTAQA541QALGLAQLAA AVPTSRGMPG TVQSGQAHLA SSPPSSQAPG ALQECPPTLA PGMTLAPVQG601TAHVVKGGAT TSSPVVAQVP AAFYMQSVHL PGKPQTLAVK RKADSEEERD DVSTLGSMLP661AKASPVAESP KVMDEKSSLG EKAESVANVN ANTPSSELVA LTPAPSVPPP TLAMVSRQMG721DSKPPQAIVK PQILTHIIEG FVIQEGAEPF PVGCSQLLKE SEKPLQTGLP TGLTENQSGG781PLGVDSPSAE LDKKANLLKC EYCGKYAPAE QFRGSKRFCS MTCAKRYNVS CSHQFRLKRK841KMKEFQEANY ARVRRRGPRR SSSDIARAKI QGKCHRGQED SSRGSDNSSY DEALSPTSPG901PLSVRAGHGE RDLGNPNTAP PTPELHGINP VFLSSNPSRW SVEEVYEFIA SLQGCQEIAE961EFRSQEIDGQ ALLLLKEEHL MSAMNIKLGP ALKICAKINV LKETTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1004 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.