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PHACTR4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PHACTR4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all PHACTR4 antibodies →
$504.00each
Catalog Number: LS-G110138-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHACTR4 (NM_023923), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP11-442N24__A.1 | PHACTR4
Origin Species
Human
NCBI GenBank
NM_023923
SwissProt
Q8IZ21

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLDSVEAGD TTPPTKRKSK FSGFGKIFKP WKWRKKKSSD KFKETSEVLE RKISMRKPRE
61ELVKRGVLLE DPEQGGEDPG KPSDAMLKNG HTTPIGNARS SSPVQVEEEP VRLASLRKAI
121PEEDLKKRLG STGSQPNSEA ESVPENVPKP PLLPPKRPLS SSHEASEGQA KDATSSGGTA
181RFIISTSITT APAATTAATS LAKTVNLSVT PSPAPRTLPA APASTNTTAT PSLTHMVPAK
241QPPIPPPKPA HRNSNPVIAE LSQAINSGTL LSKPSPPLPP KRGIPSTSVP TLESAAAITT
301KTPSDEREKS TCSMGSELLP MISPRSPSPP LPTHIPPEPP RTPPFPAKTF QVVPEIEFPP
361SLDLHQEIPQ QEDQKKEVPK RILDQNFGEP HIPSRLPPLP LHIRIQQALT SPLPMTPILE
421GSHRAHSLLF ENSDSFSEDS STLGRTRSLP ITIEMLKVPD DEEEEEQTCP STFSEEMTPT
481SVIPKLPQCL REEEEKESDS DSEGPIQYRD EEDEDESYQS ALANKVKRKD TLAMKLNHRP
541SEPELNLNSW PCKSKEEWNE IRHQIGNTLI RRLSQRPTPE ELEQRNILQP KNEADRQAEK
601REIKRRLTRK LSQRPTVAEL LARKILRFNE YVEVTDAQDY DRRADKPWTK LTPADKAAIR
661KELNEFKSSE MEVHEESKHF TRYHRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.