Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
PGCP / Aminopeptidase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101389-100
Catalog Number: LS-G101389
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PGCP(CPQ) (NM_016134), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Aminopeptidase | CPQ | LDP | LCH1 | PGCP | Ser-Met dipeptidase | Carboxypeptidase Q | Lysosomal dipeptidase
- Origin Species
- Human
- NCBI GenBank
- NM_016134
- SwissProt
- Q9Y646
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKFLIFAFFG GVHLLSLCSG KAICKNGISK RTFEEIKEEI ASCGDVAKAI INLAVYGKAQ61NRSYERLALL VDTVGPRLSG SKNLEKAIQI MYQNLQQDGL EKVHLEPVRI PHWERGEESA121VMLEPRIHKI AILGLGSSIG TPPEGITAEV LVVTSFDELQ RRASEARGKI VVYNQPYINY181SRTVQYRTQG AVEAAKVGAL ASLIRSVASF SIYSPHTGIQ EYQDGVPKIP TACITVEDAE241MMSRMASHGI KIVIQLKMGA KTYPDTDSFN TVAEITGSKY PEQVVLVSGH LDSWDVGQGA301MDDGGGAFIS WEALSLIKDL GLRPKRTLRL VLWTAEEQGG VGAFQYYQLH KVNISNYSLV361MESDAGTFLP TGLQFTGSEK ARAIMEEVMS LLQPLNITQV LSHGEGTDIN FWIQAGVPGA421SLLDDLYKYF FFHHSHGDTM TVMDPKQMNV AAAVWAVVSY VVADMEEMLP RSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
472 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.